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. 2019 Dec 13;43(4):404-419.
doi: 10.3906/biy-1907-5. eCollection 2019.

Identification of watermelon heat shock protein members and tissue-specific gene expression analysis under combined drought and heat stresses

Yasemin Çelik Altunoğlu  1 , Merve Keleş  1 , Tevfik Hasan Can  1 , Mehmet Cengiz Baloğlu  1
Affiliations

Identification of watermelon heat shock protein members and tissue-specific gene expression analysis under combined drought and heat stresses

Yasemin Çelik Altunoğlu et al. Turk J Biol. .

Abstract

Heat shock protein (Hsp) gene family members in the watermelon genome were identified and characterized by bioinformatics analysis. In addition, expression profiles of genes under combined drought and heat stress conditions were experimentally analyzed. In the watermelon genome, 39 genes belonging to the sHsp family, 101 genes belonging to the Hsp40 family, 23 genes belonging to the Hsp60 family, 12 genes belonging to the Hsp70 family, 6 genes belonging to the Hsp90 family, and 102 genes belonging to the Hsp100 family were found. It was also observed that the proteins in the same cluster in the phylogenetic trees had similar motif patterns. When the estimated 3-dimensional structures of the Hsp proteins were examined, it was determined that the α-helical structure was dominant in almost all families. The most orthologous relationship appeared to be between watermelon, soybean, and poplar in the ClaHsp gene families. For tissue-specific gene expression analysis under combined stress conditions, expression analysis of one representative Hsp gene each from root, stem, leaf, and shoot tissues was performed by real-time PCR. A significant increase was detected usually at 30 min in almost all tissues. This study provides extensive information for watermelon Hsps, and can enhance our knowledge about the relationships between Hsp genes and combined stresses.

Keywords: Citrillus lanatus(watermelon); abiotic stress; bioinformatics analysis; gene expression analysis; heat shock protein.

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Conflict of interest statement

CONFLICT OF INTEREST: none declared

Figures

Figure 1
Figure 1
The localization of ClaHsp genes on watermelon chromosomes.
Figure 2
Figure 2
Phylogenetic tree of ClaHsp100 proteins. The phylogenetic trees of the Hsp families were drawn by multiple sequence alignment using the ClustalW program via the MEGA 7 program. The maximum likelihood method (Milligan, 2003) was used when phylogenetic trees were drawn. The drawn tree was visualized in iTOL database.
Figure 3
Figure 3
Gene ontology analysis of ClaHsp proteins.
Figure 4
Figure 4
Orthologues of ClaHsp genes in other plants and divergence rates.
Figure 5
Figure 5
ClaHsp genes targeted by miRNAs.
Figure 6
Figure 6
The expression levels of ClaHsp genes in stem, root, leaf, and tendril tissues of watermelon under combined drought and temperature stresses at 0 (control), 30 min, 1 h, and 2 h.
See this image and copyright information in PMC

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