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Case Reports
. 2019 Nov 6;7(12):2449-2455.
doi: 10.1002/ccr3.2533. eCollection 2019 Dec.

Regeneration and expansion of autologous full-thickness skin through a self-propagating autologous skin graft technology

Charles W Patterson  1   2 , Matthew Stark  3 , Silpa Sharma  2 , Gerhard S Mundinger  1   2
Affiliations
Case Reports

Regeneration and expansion of autologous full-thickness skin through a self-propagating autologous skin graft technology

Charles W Patterson et al. Clin Case Rep. .

Abstract

New autologous skin regeneration technology yielded full-thickness skin as evidenced by clinical observation and skin biopsy 5 months after surgery, providing relief for debilitating split-thickness skin graft contracture in a pediatric burn case.

Keywords: autologous tissue; dermis; epidermis; full‐thickness; hair follicle; hypodermis; melanin; skin regeneration.

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Conflict of interest statement

Dr Mundinger received consulting fees from PolarityTE and is on the company's clinical advisory board. No other author has any conflict of interest to disclose, and there has been no financial support for this work, use of the technology, or manuscript preparation. Commercial Disclosure: G. Mundinger is a Clinical Advisor for PolarityTE.

Figures

Figure 1
Figure 1
A, Preoperative images of anterior chest burn scar contracture, keloid scar, and previously placed split‐thickness skin graft with region of interest shown in (B). C, Harvested skin sent for AHSC processing. D, Full‐thickness wound bed preparation for application of AHSC
Figure 2
Figure 2
Linear progression of wound healing following product application. A, Day of deployment—AHSC is spread evenly over the debrided 200 cm2 wound, B, 2 wk post‐AHSC deployment, C, 4 wk, D, 6 wk, E, 5 mo, black pen mark (circled in yellow) indicates site of biopsy, and F, 13 mo. Area treated with AHSC highlighted with white dotted lines
Figure 3
Figure 3
Polarized dermoscopic imaging of native skin (A,B) and AHSC‐regenerated skin (C,D). High‐powered fields of native skin (B) and AHSC‐regenerated skin (D) demonstrated distribution of melanin and retaining darker pigment of harvested skin. Hair (*) in AHSC‐regenerated skin (D) resemble those of native skin (B). E, Pinch testing of AHSC‐regenerated skin demonstrated pliable, noncontracted skin with functional characteristics similar to native skin
Figure 4
Figure 4
A, Stereoscopic image of punch biopsy taken at 5 mo demonstrating recapitulation of full‐thickness skin including epidermis, dermis, and hypodermal fat. B, High‐power field shows rete pegs at epidermal‐dermal junction (white arrows). C, Masson's trichrome staining demonstrating normal collagen architecture. D, Hematoxylin and eosin staining demonstrating full‐thickness skin, rete pegs (white arrows), and dermal vasculature (*) with region of interest E, showing all layers of the epidermis (SC, stratum corneum; SL, stratum lucidum, SG, stratum granulosum; SS, stratum spinosum; and SB, stratum basale). Scale bars represent 100 micrometers. F, Hematoxylin and Eosin staining of the whole section of regenerated skin biopsy demonstrating full‐thickness skin with epidermal, dermal, and hypodermal layers present 5 mo post‐AHSC therapy
See this image and copyright information in PMC

References

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