Differential response of cuticular wax and photosynthetic capacity by glaucous and non-glaucous wheat cultivars under mild and severe droughts

Abstract

Cuticular wax is known to play an important role in non-stomatal transpiration. However, support is lacking regarding the waxy phenotype for wheat breeding against drought. In this study, four wheat cultivars with different wax phenotypes (glaucous and non-glaucous types) were used to evaluate their responses to drought stress and impact on photosynthetic capability of wheat. Xinong 291 and HY 2912, with the glaucous trait, demonstrated higher diketone ratios and contents compared with Pubing 201 and Jinmai 47, which are the non-glaucous type. The cultivars HY 2912 and Jinmai 47 had 35% higher biomass than did Xinong 291 and Pubing 201 under severe drought condition. HY 2912 exhibited the highest wax load with or without drought stress. Jinmai 47 showed the highest ratio of alkane content. Among glaucous cultivars, drought-resistant HY 2912 may promote growth by decreasing water loss, increasing the diketone content, increasing the total wax load, and maintaining mesophyll and stomatal conductance. Among non-glaucous cultivars, drought-resistant Jinmai 47 may enhance growth via stomatal closure and increased mesophyll conductance and alkane ratios. The glaucous trait was not always associated with drought resistance, and correlation analysis revealed that the diketone ratio was positively related to the intercellular CO₂ concentration. These results suggest that the mechanism of drought resistance in wheat is systematically regulated by wax alteration, stomatal conductance and mesophyll conductance. Therefore, wax content and composition as well as mesophyll and stomatal regulation should be considered in the breeding and selection of drought-resistant wheat cultivars.

Keywords: Cuticular wax; Drought; Glaucous; Photosynthesis; Wheat.