Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jul;32(7):1103-5.
doi: 10.1128/AAC.32.7.1103.

Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin

Affiliations

Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin

H Hosotsubo et al. Antimicrob Agents Chemother. 1988 Jul.

Abstract

A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Appl Microbiol. 1969 Apr;17(4):497-503 - PubMed
    1. Ann Intern Med. 1969 Dec;71(6):1089-95 - PubMed
    1. Antimicrob Agents Chemother. 1977 Jan;11(1):44-6 - PubMed
    1. J Infect Dis. 1977 Mar;135(3):414-22 - PubMed
    1. Drugs. 1983 Jan;25(1):41-62 - PubMed

LinkOut - more resources