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. 1988 Jul;32(7):1103-5.
doi: 10.1128/AAC.32.7.1103.

Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin

H Hosotsubo  1 , J Takezawa, N Taenaka, K Hosotsubo, I Yoshiya
Affiliations

Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin

H Hosotsubo et al. Antimicrob Agents Chemother. 1988 Jul.

Abstract

A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.

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