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. 2020 Jan;83(1):60-66.
doi: 10.1097/JCMA.0000000000000202.

The expression of Th9 and Th22 cells in rats with cerebral palsy after hUC-MSC transplantation

Xia Niu  1 , Xiaoyan Xu  2 , Zhihua Luo  2 , De Wu  2 , Jiulai Tang  2
Affiliations

The expression of Th9 and Th22 cells in rats with cerebral palsy after hUC-MSC transplantation

Xia Niu et al. J Chin Med Assoc. 2020 Jan.

Abstract

Background: This study aimed to investigate the expression of Th9 and Th22 cells in rats with cerebral palsy (CP) after human umbilical cord-derived mesenchymal stem cell (hUC-MSC) transplantation.

Methods: First, hUC-MSCs were isolated from fresh umbilical cords and identified. Rats were divided into the normal group, CP group, and hUC-MSC transplantation group. The Morris water maze and balance beam tests were performed to evaluate the neurobehavioral ability of the rats. The levels of TNF-α, IL-6, IL-9, and IL-22 in rat brain tissues were detected by ELISA. Th9 and Th22 proportions in brain tissues were detected by flow cytometric analysis. The mRNA levels of IL-9, IL-22, PU.1, and AHR in brain tissues were determined by qRT-PCR.

Results: hUC-MSC transplantation enhanced the neurobehavioral ability of CP rats. Furthermore, Th9 and Th22 proportions were decreased in brain tissues from CP rats after hUC-MSC transplantation. The levels of proinflammatory cytokines (TNF-α and IL-6), Th9-related IL-9 and PU.1, and Th22-related IL-22 and AHR were markedly higher in brain tissues from CP rats than in brain tissues from control rats, but their levels were significantly decreased after hUC-MSC transplantation.

Conclusion: Our data indicate that Th9 and Th22 proportions are decreased in CP rats after hUC-MSC transplantation.

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Comment in

  • Mesenchymal stem cells and cerebral palsy.
    Lee FK, Lin YL, Wang PH. Lee FK, et al. J Chin Med Assoc. 2020 Apr;83(4):323-324. doi: 10.1097/JCMA.0000000000000263. J Chin Med Assoc. 2020. PMID: 31985567 No abstract available.

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