Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers
- PMID: 31906818
- DOI: 10.1080/09537104.2019.1709630
Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers
Abstract
Flow cytometry is a valuable tool in determining the phenotype and function of platelets accurately. The emergence of platelet flow cytometry in recent years provides an attractive alternative to other platelet analytical techniques, with advantages such as requiring small volumes and being highly sensitive to minimal changes in receptor function and expression. Here we present a methodical approach encompassing the stages in the development and optimization of platelet flow cytometry panels based on our extensive experience in this area.
Keywords: Flow Cytometry; Paediatrics; Panel Design and Optimization; Platelet.
Comment in
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Flow cytometry for platelets, EDTA or not EDTA: what is the answer?Platelets. 2021 Jan 2;32(1):144-145. doi: 10.1080/09537104.2020.1823361. Epub 2020 Sep 19. Platelets. 2021. PMID: 32951494 No abstract available.
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