Purification of the growth-related protein p25 of the Ehrlich ascites tumor and analysis of its isoforms
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- PMID: 3190723
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Purification of the growth-related protein p25 of the Ehrlich ascites tumor and analysis of its isoforms
Biochem Int.
1988 Aug.
Abstract
1. Two of the three isoforms of the growth-related protein p25 of the Ehrlich ascites tumor have been purified to homogeneity by giant two-dimensional polyacrylamide gel electrophoresis. 2. Antibodies raised against the isoform p25/1 react also with isoforms p25/2 and p25/3. 3. Limited tryptic digestion of p25/1 and p25/2 resulted in similar oligopeptide patterns. Corresponding oligopeptides of both isoforms have identical amino acid sequences. 4. The isoforms p25/2 and p25/3 are phosphorylated derivatives of unphosphorylated p25/1. The phosphorus is bound to serine and a further unknown phosphorylation site.
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