Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines

Affiliations

¹ Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
² Department of System Chemotherapy and Molecular Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
³ Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. takao.kataoka@kit.ac.jp.
⁴ The Center for Advanced Insect Research Promotion (CAIRP), Kyoto Institute of Technology, Kyoto, Japan. takao.kataoka@kit.ac.jp.

PMID: 31907597
DOI: 10.1007/s00418-019-01842-z

Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines

Kosuke Baba et al. Histochem Cell Biol. 2020 Apr.

. 2020 Apr;153(4):199-213.

doi: 10.1007/s00418-019-01842-z. Epub 2020 Jan 6.

Authors

Affiliations

¹ Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
² Department of System Chemotherapy and Molecular Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
³ Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. takao.kataoka@kit.ac.jp.
⁴ The Center for Advanced Insect Research Promotion (CAIRP), Kyoto Institute of Technology, Kyoto, Japan. takao.kataoka@kit.ac.jp.

PMID: 31907597
DOI: 10.1007/s00418-019-01842-z

Erratum in

Correction to: Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.
Baba K, Kuwada S, Nakao A, Li X, Okuda N, Nishida A, Mitsuda S, Fukuoka N, Kakeya H, Kataoka T. Baba K, et al. Histochem Cell Biol. 2021 Sep;156(3):295-296. doi: 10.1007/s00418-021-02010-y. Histochem Cell Biol. 2021. PMID: 34232365 No abstract available.

Abstract

Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. Rat LAMP1 fused to enhanced green fluorescent protein (EGFP) mostly accumulated at a particular cytoplasmic area and barely co-localized with LysoTracker® Red DND-99 in golden hamster kidney BHK-21 cells and Chinese hamster ovary CHO-K1 cells. Golden hamster, Chinese hamster, and human LAMP1-EGFP showed a similar intracellular distribution to rat LAMP1-EGFP in BHK-21 cells. Endogenous LAMP1 was also detected in a perinuclear area in BHK-21 cells and CHO-K1 cells, and co-localized with rat CD63-EGFP in BHK-21 cells. Moreover, rat LAMP1-DsRed-Monomer co-localized well with the human trans-Golgi network protein 2-EGFP in BHK-21 cells. These results reveal that LAMP1 predominantly localizes to the trans-Golgi network in BHK-21 cells.

Keywords: BHK-21; CHO-K1; LAMP1; Lysosome; trans-Golgi network.

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Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines

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Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines

Authors

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