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. 2020 Jan;13(1):e009791.
doi: 10.1161/CIRCIMAGING.119.009791. Epub 2020 Jan 8.

64Cu-ATSM Positron Emission Tomography/Magnetic Resonance Imaging of Hypoxia in Human Atherosclerosis

Xingyu Nie  1   2 , Andrew Elvington  3 , Richard Laforest  1 , Jie Zheng  1 , Thomas F Voller  1 , Mohamed A Zayed  4 , Dana R Abendschein  5 , Nilantha Bandara  1 , Jinbin Xu  1 , Ran Li  1 , Gwendalyn J Randolph  3 , Robert J Gropler  1   5 , Suzanne E Lapi  2   6 , Pamela K Woodard  1   2
Affiliations

64Cu-ATSM Positron Emission Tomography/Magnetic Resonance Imaging of Hypoxia in Human Atherosclerosis

Xingyu Nie et al. Circ Cardiovasc Imaging. 2020 Jan.
No abstract available

Keywords: atherosclerosis; carotid arteries; hypoxia; jugular veins; macrophage.

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Conflict of interest statement

DISCLOSURES

There is no conflict of interest to disclose.

Figures

FIGURE 1.
FIGURE 1.. Imaging of hypoxic cells in carotid atherosclerosis.
Focal 64Cu-ATSM uptake was detected in the plaque (red arrow) of the left carotid artery of a representative 69-year-old male patient as displayed in (A) 64Cu-ATSM PET/T1-weighted MR fused image and (B) 64Cu-ATSM PET only image. 64Cu-ATSM retention was also identified in the adjacent salivary glands, and a small amount of uptake is seen in muscle. (C) and (D) are the zoomed-in view of (A) and (B) with the region of interest (ROI) placed on the carotid artery (blue box). Plaque components of intraplaque hemorrhage and lipid-rich necrotic core (LRNC) are outlined in the corresponding (E) T1-weighted MRI, (F) T2-weighted MRI, (G) PD-weighted MRI, (H) TOF MRI. (I) Segmented T1-weighted MR image shows that there was one large region of hemorrhage & LRNC core comprising 25.6% of the entire plaque area. (J) Parametric map of Ktrans values color coded from 0 to 0.4 min−1 and overlaid on anatomic MR image suggest that the highly vascularized adventitia at the outer rim has the highest Ktrans values. The IHC staining of (L) PIMO for hypoxic cells, (M) CD68 for macrophages, (N) HIF-1α for hypoxia-inducible factor-1α and (O) DAPI (4′,6-diamidino-2-phenylindole) for nuclei on adjacent slices of CEA specimen slices from this patient were co-localized as shown in orange areas (red arrow) of (K) the overlaid image. Internal elastic lamina is denoted by the yellow arrow. Asterisk and star refer to the extracellular space and carotid artery lumen, respectively.
See this image and copyright information in PMC

References

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