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. 2021 Feb;28(1):35-46.
doi: 10.1111/1744-7917.12751. Epub 2020 May 25.

Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses

Jiang-Jie Li  1 , Yan Shi  1 , Gan-Lin Lin  1 , Chun-Hong Yang  1 , Tong-Xian Liu  1
Affiliations

Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses

Jiang-Jie Li et al. Insect Sci. 2021 Feb.

Abstract

Insect neuropeptides play an important role in regulating physiological functions such as growth, development, behavior and reproduction. We identified temperature-sensitive neuropeptides and receptor genes of the cotton whitefly, Bemisia tabaci. We identified 38 neuropeptide precursor genes and 35 neuropeptide receptors and constructed a phylogenetic tree using additional data from other insects. As temperature adaptability enables B. tabaci to colonize a diversity of habitats, we performed quantitative polymerase chain reaction with two temperature stresses (low = 4 °C and high = 40 °C) to screen for temperature-sensitive neuropeptides. We found many neuropeptides and receptors that may be involved in the temperature adaptability of B. tabaci. This study is the first to identify B. tabaci neuropeptides and their receptors, and it will help to reveal the roles of neuropeptides in temperature adaptation of B. tabaci.

Keywords: Bemisia tabaci; G-protein-coupled receptors; expression profiling; neuropeptide.

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Figures

Fig. 1
Fig. 1
Alignment of the precursor and mature peptide sequences of FMRFamide and Orcokinin. (A) The yellow amino acid indicates the putative signal peptides, light blue indicates the mature peptide, red indicates cleavage signals and pink indicates amidation signals. (B,C) Sequence alignment of mature peptides of FMRFamide, Orcokinin‐A and Orcokinin‐B.
Fig. 2
Fig. 2
Alignment of the precursor and mature peptide sequences of ecdysis triggering hormone (ETH). (A) Deduced amino acid sequences of Bemisia tabaci ETH. The yellow amino acid indicates the putative signal peptides, light blue indicates the mature peptide, red indicates cleavage signals and pink indicates amidation signals. (B) Sequence alignment of mature peptides of ETH.
Fig. 3
Fig. 3
Phylogenetic tree of the G‐protein‐coupled receptors (GPCRs). The neuropeptide GPCRs of Bemisia tabaci are shown in red star. Bt, B. tabaci; Bm, Bombyx mori; Dc, Diaphorina citri; Dm, Drosophila melanogaster; Nl, Nilaparvata lugens; Tc, Tribolium castaneum; Zn, Zootermopsis nevadensis.
Fig. 4
Fig. 4
The expression of neuropeptide genes under temperature stress in Bemisia tabaci. Data are presented as means ± SE based on three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, independent samples t‐test).
Fig. 5
Fig. 5
The expression of neuropeptide receptor genes under temperature stress in Bemisia tabaci. Data are presented as means ± SE based on three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, independent samples t‐test).
See this image and copyright information in PMC

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