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. 2020 May;226(4):1029-1041.
doi: 10.1111/nph.16417. Epub 2020 Feb 14.

DEK1 displays a strong subcellular polarity during Physcomitrella patens 3D growth

Pierre-François Perroud  1 , Rabea Meyberg  1 , Viktor Demko  2 , Ralph S Quatrano  3 , Odd-Arne Olsen  4 , Stefan A Rensing  1   5   6
Affiliations
Free article

DEK1 displays a strong subcellular polarity during Physcomitrella patens 3D growth

Pierre-François Perroud et al. New Phytol. 2020 May.
Free article

Abstract

Defective Kernel 1 (DEK1) is genetically at the nexus of the 3D morphogenesis of land plants. We aimed to localize DEK1 in the moss Physcomitrella patens to decipher its function during this process. To detect DEK1 in vivo, we inserted the tdTomato fluorophore into PpDEK1 gene locus. Confocal microscopy coupled with the use of time-gating allowed the precise DEK1 subcellular localization during 3D morphogenesis. DEK1 localization displays a strong polarized signal, as it is restricted to the plasma membrane domain between recently divided cells during the early steps of 3D growth development as well as during the subsequent vegetative growth. The signal furthermore displays a clear developmental pattern because it is only detectable in recently divided and elongating cells. Additionally, DEK1 localization appears to be independent of its calpain domain proteolytic activity. The DEK1 polar subcellular distribution in 3D tissue developing cells defines a functional cellular framework to explain its role in this developmental phase. Also, the observation of DEK1 during spermatogenesis suggests another biological function for this protein in plants. Finally the DEK1-tagged strain generated here provides a biological platform upon which further investigations into 3D developmental processes can be performed.

Keywords: 3D development; DEK1; Physcomitrella patens; cell polarity; spermatozoid; subcellular localization.

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References

    1. Ako AE, Perroud P-F, Innocent J, Demko V, Olsen O-A, Johansen W. 2017. An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing. Scientific Reports 7: 5111.
    1. Amanda D, Doblin MS, Galletti R, Bacic A, Ingram GC, Johnson KL. 2016. DEFECTIVE KERNEL1 (DEK1) Regulates cell walls in the leaf epidermis. Plant Physiology 172: 2204-2218.
    1. Ansari AM, Ahmed AK, Matsangos AE, Lay F, Born LJ, Marti G, Harmon JW, Sun Z. 2016. Cellular GFP toxicity and immunogenicity: potential confounders in in vivo cell tracking experiments. Stem Cell Reviews and Reports 12: 553-559.
    1. Arthur JSC, Gauthier S, Elce JS. 1995. Active site residues in m-calpain: identification by site-directed mutagenesis. FEBS Letters 368: 397-400.
    1. Ashton NW, Raju MVS. 2014. The distribution of gametangia on gametophores of Physcomitrella (Aphanoregma) patens in culture. Journal of Bryology 22: 9-12.

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