A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities
- PMID: 31918
- DOI: 10.1016/0005-2744(78)90363-7
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities
Abstract
A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.
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