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. 1978 Dec 8;527(2):497-500.
doi: 10.1016/0005-2744(78)90363-7.

A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities

L A Otten, R A Schilperoort

A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities

L A Otten et al. Biochim Biophys Acta. .

Abstract

A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.

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