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Comment
. 2020 Jan 10;295(2):298.
doi: 10.1074/jbc.L119.011910.

Specificity of anti-MYC antibodies

Giorgio Cattoretti  1
Affiliations
Comment

Specificity of anti-MYC antibodies

Giorgio Cattoretti. J Biol Chem. .
No abstract available

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Conflict of interest statement

The author declares that he has no conflicts of interest with the contents of this article.

Figures

Figure 1.
Figure 1.
Validation of MYC antibodies by flow cytometry. A, exponentially growing (control) or TPA growth-arrested U937 cells are stained by flow cytometry with a negative control (gray shadow) or color-coded anti-MYC mouse monoclonal antibodies. C-33 has a bimodal distribution in growing U937 cells, reflecting the S-phase content (bottom DNA ploidy histograms). The other antibodies do not distinguish growing versus growth-arrested cells in this assay. B, the treated and untreated U937 cells of A were fixed, embedded, and stained with an Rb anti-MYC antibody (sc-764), showing positive staining in growing cells only. Northern blotting of the same cells' RNA extract probed for MYC and a loading control is shown at the right. This research was originally published in the Journal of Pathology. Cattoretti, G. MYC expression and distribution in normal mature lymphoid cells. J. Pathol. 2012; 229:430–440. © Wiley.
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