Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Apr;117(4):1187-1203.
doi: 10.1002/bit.27269. Epub 2020 Jan 23.

Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production

Matthew N Henry  1 , Michael A MacDonald  2 , Camila A Orellana  1 , Peter P Gray  1 , Marianne Gillard  1 , Kym Baker  2   3 , Lars K Nielsen  1   2   4   5 , Esteban Marcellin  1   2   4 , Stephen Mahler  2 , Verónica S Martínez  2
Affiliations
Review

Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production

Matthew N Henry et al. Biotechnol Bioeng. 2020 Apr.

Abstract

Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.

Keywords: CHO cell line engineering; apoptosis; biopharmaceutical production; programmed cell death.

PubMed Disclaimer

References

REFERENCES

    1. Aboulaich, N., Chung, W. K., Thompson, J. H., Larkin, C., Robbins, D., & Zhu, M. (2014). A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies. Biotechnology Progress, 30(5), 1114-1124. https://doi.org/10.1002/btpr.1948
    1. Al-Rubeai, M., & Sing, R. P. (1999). Apoptosis in cell culture. Biochemical Engineering, 9, 152-156.
    1. Altieri, D. C. (2010). Survivin and IAP proteins in cell-death mechanisms. Biochemical Journal, 430(2), 199-205. https://doi.org/10.1042/BJ20100814
    1. Arden, N., & Betenbaugh, M. J. (2004). Life and death in mammalian cell culture: Strategies for apoptosis inhibition. Trends in Biotechnology, 22(4), 174-180. https://doi.org/10.1016/j.tibtech.2004.02.004
    1. Arden, N., Majors, B. S., Ahn, S., Oyler, G., & Betenbaugh, M. J. (2006). Inhibiting the apoptosis pathway using MDM2 in mammalian cell cultures. Biotechnology and Bioengineering, 97(3), 601-614. https://doi.org/10.1002/bit.21254

Publication types

Substances

LinkOut - more resources