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. 2019 Oct 1;12(10):3839-3846.
eCollection 2019.

Expression of ARHGAP10 correlates with prognosis of prostate cancer

Hua Gong  1 , Xingyi Chen  2 , Yongcao Jin  2 , Jiasun Lu  2 , Yuanjue Cai  3 , Ouyang Wei  3 , Jun Zhao  3 , Wenyuan Zhang  3 , Xiaofei Wen  2 , Yuemin Wang  2 , Weihua Chen  2   3
Affiliations

Expression of ARHGAP10 correlates with prognosis of prostate cancer

Hua Gong et al. Int J Clin Exp Pathol. .

Abstract

Prostate cancer is one of the most common malignancies in men worldwide. Altered expression of ARHGAP10, a member of the Rho GTPase activating protein (RhoGAP) family, has been found in several human cancers. However, its clinical significance in prostate cancer remains unknown. In the current study, we found that mRNA levels of ARHGAP10 were significantly higher in prostate cancer tissues than in the non-cancerous controls. Gene set enrichment analysis (GSEA) revealed that ARHGAP10 expression was negatively correlated with the Wnt signaling pathway. Immunohistochemical staining results showed that 62.2% (56/90) and 65.5% (59/90) of prostate cancer tissues displayed low expression of ARHGAP10 and high expression of β-catenin, respectively. ARHGAP10 protein expression was significantly correlated with histologic grade (P < 0.0001), tumor stage (P = 0.0298), preoperative prostate specific antigen level (P = 0.0261), vital status (P = 0.0017) and β-catenin expression (P < 0.0001). Kaplan-Meier survival analysis indicated that patients with low levels of ARHGAP10 and high levels of β-catenin had poor overall survival. Multivariate analyses revealed that ARHGAP10 and β-catenin expression was independent prognostic factor for prostate cancer. In summary, the current study suggests that ARHGAP10 in association with β-catenin may play a role in the development of prostate cancer and serve as a prognostic factor for this disease.

Keywords: ARHGAP10; prognosis; prostate cancer; β-catenin.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Decreased expression of ARHGAP10 mRNA in prostate cancer tissues. ARHGAP10 mRNA expression was analyzed by Student t test based on two public available datasets, GSE55945 (A) and TCGA dataset (B).
Figure 2
Figure 2
Protein expression of ARHGAP10 and β-catenin in prostate cancer tissues. A. The GSEA result showed the correlation of ARHGAP10 expression and the Wnt signaling pathways. NES, normalized enrichment score; FDR, false discovery rate. B. Protein was extracted from eight pairs of prostate cancer (T1-T8) and non-cancerous tissues (N1-N8) with ice-cold RIPA buffer. Western blotting analysis was then performed to detect the protein levels of ARHGAP10 and β-catenin, and GAPDH served as a loading control. Representative images and quantitative analysis are shown.
Figure 3
Figure 3
Immunohistochemical staining was conducted to detect the expression of ARHGAP10 (A) and β-catenin (B) in prostate cancer and non-cancerous tissues. Scale bars: 100 μm.
Figure 4
Figure 4
Kaplan-Meier survival curves were generated to analyze patients in relationship to ARHGAP10 expression (A), β-catenin expression (B), and ARHGAP10 and β-catenin coexpression (C).
See this image and copyright information in PMC

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