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. 2020 Jan 6;10(1):89.
doi: 10.3390/ani10010089.

Verification and Analysis of Sheep Tail Type-Associated PDGF-D Gene Polymorphisms

Qing Li  1 , Zengkui Lu  2 , Meilin Jin  1 , Xiaojuan Fei  1 , Kai Quan  3 , Yongbin Liu  4 , Lin Ma  1 , Mingxing Chu  1 , Huihua Wang  1 , Caihong Wei  1
Affiliations

Verification and Analysis of Sheep Tail Type-Associated PDGF-D Gene Polymorphisms

Qing Li et al. Animals (Basel). .

Abstract

The aim of this study was to examine the correlation between the platelet-derived growth factor-D (PDGF-D) gene and sheep tail type character and explore the potential underlying mechanism. A total of 533 sheep were included in this study. Polymorphic sites were examined by Pool-seq, and individual genotype identification and correlation analysis between tail type data were conducted using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) method. JASPART website was used to predict transcription factor binding sites in the promoter region with and without PDGF-D gene mutation. The effect of PDGF-D on adipogenic differentiation of sheep preadipocytes was investigated. Two single nucleotide polymorphism sites were identified: g.4122606 C > G site was significantly correlated with tail length, and g.3852134 C > T site was significantly correlated with tail width. g.3852134 C > T was located in the promoter region. Six transcription factor binding sites were eliminated after promoter mutation, and three new transcription factor binding sites appeared. Expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) and lipoproteinlipase (LPL) were significantly up-regulated upon PDGF-D overexpression. Oil red O staining showed increased small and large oil drops in the PDGF-D overexpression group. Together these results indicate the PDGF-D gene is an important gene controlling sheep tail shape and regulating sheep tail fat deposition to a certain degree.

Keywords: PDGF-D; SNPs; preadipocytes; sheep; tail type.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
PDGF-D gene pool sequencing and genotyping results. (A) Site of g.4122606 C > G. (B) Site of g.3852134 C > T. Yellow region, blue region, and green region represent different genotypes. Numbers in brackets indicate number of individuals of the three genotypes.
Figure 2
Figure 2
mRNA expression of the PPARγ and LPL genes. (A) mRNA expression of the PPARγ gene. (B) mRNA expression of the LPL gene. * p < 0.05, ** p < 0.01, NS: no significant difference.
Figure 3
Figure 3
Oil red O staining when overexpressing the PDGF-D gene in sheep preadipocytes after 7 days of induced differentiation. (A) Control group. (B) PDGF-D overexpressed group.
See this image and copyright information in PMC

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