Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jan 9;21(2):431.
doi: 10.3390/ijms21020431.

Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

Pietro Gentile  1 , Claudio Calabrese  2 , Barbara De Angelis  1 , Laura Dionisi  3 , Jacopo Pizzicannella  4 , Ashutosh Kothari  5 , Domenico De Fazio  6 , Simone Garcovich  7
Affiliations

Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

Pietro Gentile et al. Int J Mol Sci. .

Abstract

Autologous therapies using platelet-rich plasma (PRP) need meticulous preparation-currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The aim of this review is to use existing PQSs and PQLs to report the in vivo/in vitro results obtained by using different Kits, that utilise different procedures (classified as Closed-Technique and Opened-Technique) to isolate autologous human activated (AA-PRP) or non-activated PRP (A-PRP). PQSs included the volumes of blood collected as well as the reagents used, the time/gravity of centrifugation, and the duration, temperature and tilt level/speed of centrifugation. PQLSs included the use of Calcium Chloride CaCl2, Kit weight, transparency of Kit components, the maintenance of a closed sterile processing environment and the use of a small centrifuge. Eight CE marked devices for PRP extraction were evaluated: Angel®, Biomed®, Cascade® and Selphyl®, Mag-18®, i-Stem®, MyCells® and Regenlab®. Using a Kit with the PQSs and PQLSs described in this study enables the isolation of A-PRP, thereby meeting consensus quality criteria. As our understanding of Critical Quality Attributes (CQAs) of A-PRP continues to evolve, especially with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully help isolate A-PRP of desired CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed.

Keywords: A-PRP; PRP; PRP kit; PRP procedures; activated PRP; autologous platelet-rich plasma; non-activated PRP; platelet-rich plasma; regenerative plastic surgery.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

References

    1. Scioli M.G., Bielli A., Gentile P., Cervelli V., Orlandi A. Combined treatment with platelet-rich plasma and insulin favours chondrogenic and osteogenic differentiation of human adipose-derived stem cells in three-dimensional collagen scaffolds. J. Tissue Eng. Regen. Med. 2017;11:2398–2410. doi: 10.1002/term.2139. - DOI - PubMed
    1. Cervelli V., Lucarini L., Spallone D., Palla L., Colicchia G.M., Gentile P., De Angelis B. Use of platelet-rich plasma and hyaluronic acid in the loss of substance with bone exposure. Adv. Ski. Wound Care. 2011;24:176–181. doi: 10.1097/01.ASW.0000396302.05959.d3. - DOI - PubMed
    1. Gentile P., Bottini D.J., Spallone D., Curcio B.C., Cervelli V. Application of platelet-rich plasma in maxillofacial surgery: Clinical evaluation. J. Craniofacial Surg. 2010;2:900–904. doi: 10.1097/SCS.0b013e3181d878e9. - DOI - PubMed
    1. Gentile P., Cole J.P., Cole M.A., Garcovich S., Bielli A., Scioli M.G., Orlandi A., Insalaco C., Cervelli V. Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems. Int. J. Mol. Sci. 2017;18:408. doi: 10.3390/ijms18020408. - DOI - PMC - PubMed
    1. Harrison P., Subcommittee on Platelet Physiology The use of platelets in regenerative medicine and proposal for a new classification system: Guidance from the SSC of the ISTH. J. Thromb. Haemost. 2018;16:1895–1900. doi: 10.1111/jth.14223. - DOI - PubMed