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Comment
. 2020 Mar;32(3):543-546.
doi: 10.1105/tpc.19.00821. Epub 2020 Jan 14.

Reply: The Revised Model for Chloroplast Protein Import

Masato Nakai  1
Affiliations
Comment

Reply: The Revised Model for Chloroplast Protein Import

Masato Nakai. Plant Cell. 2020 Mar.
No abstract available

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Figures

Figure 1.
Figure 1.
Improved Method to Purify and Identify Translocation Machinery Components (A) Translocation intermediates are arrested under low [ATP] in in vitro experiments. Proteins identified by immunoblot analyses in our studies (Kikuchi et al., 2013, 2018) are shown. (B) LC-MS/MS determination of purified translocation intermediate-associating components (adapted from Kikuchi et al., 2018, Table 1). Areas of circles are depicted in proportion to the observed MS spectral counts (shown under protein names) except for those not detected. For TOC, TIC, and Ycf2/FtsHi complexes, total spectral counts of respective constituents are combined (shown under circles). *, Tic20 has been known as an extremely difficult protein to be detected by MS but was clearly detected by immunoblot analysis as well as by silver staining (Kikuchi et al., 2013), as indicated in (A).
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References

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