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. 2020 Jan;21(1):e15.
doi: 10.4142/jvs.2020.21.e15.

Restoration of the inflammatory gene expression by horse oil in DNCB-treated mice skin

Jae Chul Lee  1 , Ga Ryoung Park  1 , Byoung Soo Choi  1 , Youngjae Lee  1 , Chang Hoon Han  2
Affiliations

Restoration of the inflammatory gene expression by horse oil in DNCB-treated mice skin

Jae Chul Lee et al. J Vet Sci. 2020 Jan.

Abstract

The present study evaluated the anti-inflammatory effect of horse oil in 2, 4-dinitrochlorobenzene (DNCB)-treated BALB/c mice. After the application of DNCB, the mice showed atopic dermatitis symptoms, including severe erythema, hemorrhage, and erosion, whereas those symptoms were alleviated by treatment with horse oil. To explain the anti-dermatitis effect of horse oil, the gene expression levels in the healing process in dorsal skin were observed using a cDNA microarray. The cDNA microarray analysis revealed that the expression levels of 30 genes related to the inflammation, including Ccr1, Ccr2, Ccl20, Anxa1, and Hc genes, were up-regulated (higher than 2.0-fold) in the DNCB group compared to the levels in the control group, whereas the levels were restored to the control level in the DNCB + horse oil-treated group. In contrast, the gene expression levels of 28 genes related to inflammation, including chemokine genes Ccl5, Ccl7, Ccl8, Cxcl10, and Cxcl13 genes, were down-regulated (lower than 0.5-fold) in the DNCB group compared to the levels in the control group, whereas the levels were restored to the control level in the DNCB + horse oil-treated group. Overall, the results show that horse oil restores the expression levels of genes related to inflammation that were perturbed by DNCB treatment.

Keywords: Anti-inflammatory effect; DNCB-treated mice; cDNA microarray analysis; chemokine genes; horse oil.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1. The anti-atopic dermatitis effect of horse oil in DNCB-treated BALB/c mice. Skin lesions in the (A) control, (B) DNCB, (C) DNCB + AB, and (D) DNCB + horse oil treatment groups are shown.
DNCB, 2, 4-dinitrochlorobenzene; AB, Atobarrier lotion.
Fig. 2
Fig. 2. Isolation of RNAs from the skin of mice. RNAs were isolated from the skin of control-, DNCB-, DNCB + AB-, and DNCB + horse oil-treated mice. (A) Migration pattern (electrophoretic trace), and (B) peak pattern (electropherogram) of RNAs are shown.
DNCB, 2, 4-dinitrochlorobenzene; AB, Atobarrier lotion.
Fig. 3
Fig. 3. Scanned image of cDNA microarray chips. After labeling of cRNA, samples from the control-, DNCB-, DNCB + AB-, and DNCB + horse oil-treated mice were hybridized onto mouse oligo microarray slides, which were then scanned and analyzed.
DNCB, 2, 4-dinitrochlorobenzene; AB, Atobarrier lotion.
Fig. 4
Fig. 4. GO analysis of DNCB-treated mouse skin applied with/without AB or horse oil as a percentage of the total significant. The transcripts of the DNCB-treated mouse skin applied with/without AB or horse oil were classified into GO categories of (A) DNCB/control, (B) DNCB + AB/control, and (C) DNCB + horse oil/control based on their GO terms as a percentage of the total significant.
GO, gene ontology; DNCB, 2, 4-dinitrochlorobenzene; AB, Atobarrier lotion.
Fig. 5
Fig. 5. PPI network constructed for DEGs identified in mouse skin treated with DNCB and horse oil. Representative image of the PPI network is shown. Bold lines represent the strongly connected genes associated with inflammation. Different colors indicate DEGs; red indicates up-regulated gene expression and blue indicates down-regulated gene expression.
PPI, protein-protein interaction; DEG, differentially expressed gene; DNCB, 2, 4-dinitrochlorobenzene.
Fig. 6
Fig. 6. Summary of the relationships among chemokine ligands and receptors that were up- or down-regulated by DNCB treatment and restored by horse oil treatment.
DNCB, 2, 4-dinitrochlorobenzene.
See this image and copyright information in PMC

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