Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Apr;17(2):491-507.
doi: 10.1111/iwj.13305. Epub 2020 Jan 15.

Preparation of placental tissue transplants and their application in skin wound healing and chosen skin bullous diseases - Stevens-Johnson syndrome and toxic epidermal necrolysis treatment

Agnieszka Klama-Baryła  1 , Ewa Rojczyk  2 , Diana Kitala  1 , Wojciech Łabuś  1 , Wojciech Smętek  1 , Katarzyna Wilemska-Kucharzewska  3 , Marek Kucharzewski  2
Affiliations
Review

Preparation of placental tissue transplants and their application in skin wound healing and chosen skin bullous diseases - Stevens-Johnson syndrome and toxic epidermal necrolysis treatment

Agnieszka Klama-Baryła et al. Int Wound J. 2020 Apr.

Abstract

Unique properties of amniotic membrane make it a promising source for tissue engineering and a clinically useful alternative for patients suffering from chronic wounds including, for example, ulcers, burns, ocular surface damages and wounds occurring in the course of bullous diseases like stevens-johnson syndrome and toxic epidermal necrolysis. Its use has many advantages over standard wound care, as it contains pluripotent cells, nutrients, anti-fibrotic and anti-inflammatory cytokines, growth factors and extracellular matrix (ECM) proteins. Placental tissues can be prepared as a medical component, an advanced therapy medicinal product or a tissue graft. In addition to basic preparation procedures such as washing, rinsing, cutting, drying and sterilisation, there are many optional steps such as perforation, crosslinking and decellularisation. Finally, transplants should be properly stored-in cryopreserved or dehydrated form. In recent years, many studies including basic science and clinical trials have proven the potential to expand the use of amniotic membrane and amnion-derived cells to the fields of orthopaedics, dentistry, surgery, urology, vascular tissue engineering and even oncology. In this review, we discuss the role of placental tissues in skin wound healing and in the treatment of various diseases, with particular emphasis on bullous diseases. We also describe some patented procedures for placental tissue grafts preparation.

Keywords: amnion; healing; placenta; wound.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Properties of the amniotic membrane and its influence on skin wound healing process
Figure 2
Figure 2
The scheme of the basic procedure of amniotic membrane transplants preparation
Figure 3
Figure 3
Removal of necrotic epidermis (A,B), leaving blebs (C,D)
Figure 4
Figure 4
Clinical effect of the use of amniotic transplants in TEN: A, condition of the patient during the first days of hospitalisation. B, allogenic amnion transplantation. C, condition of the patient on the day of discharge from the intensive care unit
See this image and copyright information in PMC

References

    1. Benirschke K, Burton GJ, Baergen RN. Pathology of the Human Placenta. New York, NY: Springer‐Verlag Berlin Heidelberg; 2012.
    1. Gude NM, Roberts CT, Kalionis B, King RG. Growth and function of the normal human placenta. Thromb Res. 2004;114(5–6):397‐407. - PubMed
    1. Chen CP, Aplin JD. Placental extracellular matrix: gene expression, deposition by placental fibroblasts and the effect of oxygen. Placenta. 2003;24(4):316‐325. - PubMed
    1. Underwood MA, Gilbert WM, Sherman MP. Amniotic fluid: not just fetal urine anymore. J Perinatol. 2005;25(5):341‐348. - PubMed
    1. Nyman E, Huss F, Nyman T, Junker J, Kratz G. Hyaluronic acid, an important factor in the wound healing properties of amniotic fluid: in vitro studies of re‐epithelialisation in human skin wounds. J Plast Surg Hand Surg. 2013;47(2):89‐92. - PubMed

LinkOut - more resources