Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Feb;97(2):171-183.
doi: 10.1002/cyto.a.23964. Epub 2020 Jan 16.

An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä  1 , Hanna Ulrich  2 , Susi Dürr  1 , Dominik Soll  1 , Lynette Henkel  1 , Corinne Angerpointner  1 , Julia Ritter  2 , Sabine Przibilla  3 , Herbert Stadler  4   5 , Manuel Effenberger  1 , Dirk H Busch  1   6   7 , Matthias Schiemann  1
Affiliations
Free article

An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä et al. Cytometry A. 2020 Feb.
Free article

Abstract

Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

Keywords: MAPK; T cells; cell purification; cell sorting; cell stress; flow cytometry cell sorting; p38 activation; traceless affinity cell selection.

PubMed Disclaimer

Comment in

References

Literature Cited

    1. Saba-El-Leil MK, Fremin C, Meloche S. Redundancy in the world of MAP kinases: All for one. Front Cell Dev Biol 2016;4:67. https://doi.org/10.3389/fcell.2016.00067
    1. Nishimoto S, Nishida E. MAPK signalling: ERK5 versus ERK1/2. EMBO Rep 2006;7:782-786. https://doi.org/10.1038/sj.embor.7400755
    1. Donnelly N, Gorman AM, Gupta S, Samali A. The eIF2alpha kinases: Their structures and functions. Cell Mol Life Sci 2013;70(3):493-3,511. https://doi.org/10.1007/s00018-012-1,252-6
    1. Stemberger C, Huster KM, Koffler M, Anderl F, Schiemann M, Wagner H, Busch DH. A single naive CD8+ T cell precursor can develop into diverse effector and memory subsets. Immunity 2007;27:985-997. https://doi.org/10.1016/j.immuni.2007.10.012
    1. Graef P, Buchholz VR, Stemberger C, Flossdorf M, Henkel L, Schiemann M, Drexler I, Höfer T, Riddell SR, Busch DH. Serial transfer of single-cell-derived immunocompetence reveals stemness of CD8(+) central memory T cells. Immunity 2014;41:116-126. https://doi.org/10.1016/j.immuni.2014.05.018

Publication types

Substances

LinkOut - more resources