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. 2019 Aug 30;7(19):3127-3131.
doi: 10.3889/oamjms.2019.793. eCollection 2019 Oct 15.

Antimicrobial Activity of Terpenoids Extracted from Annona muricata Seeds and its Endophytic Aspergillus niger Strain SH3 Either Singly or in Combination

Tahany Abdel-Rahman  1 , Amal-Saad Hussein  2 , Safia Beshir  2 , Ahmed R Hamed  3 , Eman Ali  1 , Sara S El-Tanany  2
Affiliations

Antimicrobial Activity of Terpenoids Extracted from Annona muricata Seeds and its Endophytic Aspergillus niger Strain SH3 Either Singly or in Combination

Tahany Abdel-Rahman et al. Open Access Maced J Med Sci. .

Abstract

Background: Annona muricata (Soursop) has an antimicrobial activity toward various pathogenic microorganisms which support its ethnomedicinal for the treatment of many infectious diseases.

Aim: Aim of the present study to evaluate the relation between antimicrobial activities of terpenoids extracted from different soursop parts with the isolated endophytic fungi.

Methods: Endophytic fungal species of pulp and peel of Annona fruit along with those of seeds were isolated. Salkowski test was used for qualitative screening of terpenoids in plant and the isolated endophytic Aspergillus niger strain SH3.

Results: Endophytic A. niger strain SH3 and Annona seed extract showed high terpenoid content indicated by the high intensity of reddish-brown colour. GC/Mass analysis revealed six compounds of terpenoids from endophytic A. niger strain SH3 extract and four compounds from seed extract with different retention times. The antimicrobial assay was performed using A. niger strain SH3 extract and Annona seed extract singly or in combinations against S. aureus, P. aeruginosa, E. coli and C. albicans.

Conclusion: The results revealed the significant antimicrobial activity of both extracts. However, the combined extract showed some reduction in antimicrobial activity which could be attributed to the antagonistic effect exhibited by their constituents.

Keywords: Annona muricate; Antimicrobial Activity; Combined extract; Endophytic Fungi and Terpenoids.

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Figures

Figure 1
Figure 1
a) Frequency percentage of endophytic fungal species isolated from different parts of A. muricate; b) Total isolates of endophytic fungi isolated from different parts of A.muricata; c) Relative activity % of A. niger strain SH3 and seed extracts either singly or in combination
See this image and copyright information in PMC

References

    1. Coria-Téllez AV, Montalvo-Gónzalez E, Yahia EM, Obledo-Vázquez EN. Annona muricata:a comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian J Chem. 2016;1:1–4.
    1. Sandhu S, Gupta D, Mandal R, Puttey SJ. Screening of endophytic fungi isolated from some medicinal plants in Jabalpur region for antibacterial activity. World J Pharm Pharm Sci. 2014;3:1655–1666.
    1. Tan RX, Zou WX. Endophytes:a rich source of functional metabolites. Nat Prod Rep. 2001;8:448–459. https://doi.org/10.1039/b100918o PMid:11548053. - PubMed
    1. Katoch M, Singh G, Sharma S, Gupta N, Sangwan PL, Saxena AK. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae) BMC Complement Altern Med. 2014;14:1–8. https://doi.org/10.1186/1472-6882-14-52 PMid:24512530 PMCid:PMC3930298. - PMC - PubMed
    1. Nath A, Joshi SR. Ultrastructural effect on mastitis pathogens by extract of endophytic fungi associated with ethnoveterinary plant, Hibiscus sabdariffa L. J Micro Ultra. 2015;3:38–43. https://doi.org/10.1016/j.jmau.2014.10.001 PMid:30023180 PMCid:PMC6014217. - PMC - PubMed

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