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. 2018 Jul 1;11(7):3294-3301.
eCollection 2018.

Roles of PRF and IGF-1 in promoting alveolar osteoblast growth and proliferation and molecular mechanism

Xiaoyu Li  1 , Jinfeng Yao  2 , Jing Wu  1 , Xinya Du  1 , Wei Jing  3 , Lei Liu  3
Affiliations

Roles of PRF and IGF-1 in promoting alveolar osteoblast growth and proliferation and molecular mechanism

Xiaoyu Li et al. Int J Clin Exp Pathol. .

Abstract

Background: Fibrin and cytokines in platelet-rich fibrin (PRF) can be combined into a powerful biological scaffold, which is an integrated reservoir of growth factors involved in tissue regeneration. Insulin-like growth factor-1 (IGF-1) is a kind of effective mitogenic protein, which can enhance osteogenic differentiation of periodontal ligament fibroblasts. However, whether PRF and IGF-1 can stimulate the osteogenic differentiation and osteogenesis of human periodontal ligament stem cells (PDLSCs) remains unclear. This study aims to investigate the osteogenic capability of PDLSCs in vitro and in vivo after being separated from human PDL tissues, purified with STRO-1 and treated with PRF and IGF-1. Methods: The proliferative capabilities of PDLSCs under different conditions were analyzed via methyl thiazolyl tetrazolium (MTT), growth curve, alkaline phosphatase activity, reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. Results: PRF and IGF-1 significantly promoted the growth, proliferation and differentiation of PDLSCs, up regulated the expressions of Runt-related transcription factor 2 (RUNX2), osterix (OSX) and osteocalcin (OCN), phosphorylated extracellular signal-regulated kinase (ERK) and phosphorylated c-Jun N-terminal kinase (JNK) in stem cells. Conclusion: Our data indicate that PRF and IGF-1 facilitate the proliferation of alveolar osteoblast via the activation of the mitogen-activated protein kinase (MAPK) signaling pathway.

Keywords: IGF-1; PRF; molecular mechanism; osteoblast; proliferation.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Effects of PRF and IGF-1 on in-vitro proliferation of PDLSCs (n=6).
Figure 2
Figure 2
ALP staining of PDLSCs under different culture conditions. **P<0.01, n=6.
Figure 3
Figure 3
Analysis of RUNX2, OSX and OCN mRNA expressions via real-time RT-PCR. GAPDH is used as an internal control, and the results are described as the fold change relative to the GAPDH. Data are presented as mean ± SD. **P<0.01, *P<0.05, n=6.
Figure 4
Figure 4
Analysis of RUNX2, OSX and OCN protein expressions under different conditions via Western blotting.
Figure 5
Figure 5
Percentages of RUNX2, OSX and OCN positive cells in treated group and non-treated group. **P<0.01, *P<0.05, n=20.
Figure 6
Figure 6
Effects of PRF and IGF-1 on MAPK pathway in PDLSCs.
See this image and copyright information in PMC

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