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. 2020 May;95(5):457-464.
doi: 10.1111/tan.13806. Epub 2020 Feb 3.

HLA-E genotyping and its relevance in kidney transplantation outcome

Bruna L M de Miranda  1 , Geórgia F Gelmini  1 , Matilde Risti  1 , Vanessa Hauer  1 , José Samuel da Silva  1 , Valéria M M S Roxo  1 , Maria da Graça Bicalho  1 , Danielle Malheiros  2
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HLA-E genotyping and its relevance in kidney transplantation outcome

Bruna L M de Miranda et al. HLA. 2020 May.

Abstract

HLA-E, a class I nonclassical HLA molecule, is expressed in all tissues and is involved in the regulation of both innate (by interaction with the CD94/NKG2 receptor expressed mainly in NK cells) and adaptive immunity (by interaction with T CD8+ cells), suggesting a possible role in the solid organ transplantation context. Transplanted patients with chronic kidney disease and their respective donors (N = 107 pairs) were genotyped for exons 2 and 3 of the HLA-E locus by sequence-based typing (SBT). Groups' genotype frequencies were compared regarding episodes of clinical rejection by global G test, and binary logistic regression was made to demonstrate the contribution of genetic variables vs epidemiological variables. Comparisons of donors' genotype frequencies showed significant differences (P = .0230), revealing a protective profile of E*01:01/*01:01 compared to the other genotypes (P = .0099; OR = 0.3088; CI [95%] = 0.1333-0.7157). The same happened when the aforementioned genotype was combined with the E*01:01/*01:01 recipients' genotype (P = .0065; OR = 0.1760; CI [95%] = 0.0517-0.5987). A binary logistic regression analysis was performed, and, of all variables considered, only two were included in the resulting model (P = .007; R2 Cox and Snell = 0.243; R2 Nagelkerke = 0.328)- "End-Stage Renal Disease" and "HLA class II Mismatches." A protective profile (E*01:01/*01:01) was observed between the recipients and donors, suggesting a possible impact of the HLA-E genotype in rejection episodes.

Keywords: HLA-E; binary logistic regression; kidney transplantation; rejection.

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References

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