Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Aug 1;10(1):36-44.
doi: 10.1016/j.jtcme.2019.08.001. eCollection 2020 Jan.

Microarray analysis of genes from animals treated with a traditional formulation ChandraprabhaVati reveals its therapeutic targets

Pratheppa Rajagopal  1   2 , David Raj Chellappan  3   2 , Sriram Sridharan  3   2 , Brindha Pemiah  3   2 , Sridharan Krishnaswamy  2 , Swaminathan Sethuraman  1   2 , KalpoondiRajan Sekar  1   2 , Uma Maheswari Krishnan  1   2
Affiliations

Microarray analysis of genes from animals treated with a traditional formulation ChandraprabhaVati reveals its therapeutic targets

Pratheppa Rajagopal et al. J Tradit Complement Med. .

Erratum in

  • Erratum for previously published articles.
    [No authors listed] [No authors listed] J Tradit Complement Med. 2020 Sep 28;10(6):605. doi: 10.1016/j.jtcme.2020.09.001. eCollection 2020 Nov. J Tradit Complement Med. 2020. PMID: 33134138 Free PMC article.

Abstract

Background: Traditional medicinal preparations have not received global acceptance, and their therapeutic benefits remain disputed due to lack of scientific evidence on their mechanism of action. Microarray analysis has emerged as a powerful technique that can aid in understanding the complex signaling networks activated by these formulations and thereby assess their beneficial as well as adverse effects.

Aim: The present work aims to investigate the differential influence of ChandraprabhaVati, Ayurvedic formulation used in the treatment of diabetes, anemia, urinary, respiratory, skin and liver disorders.

Materials and methods: The RNA from the liver of rats treated with different doses of ChandraprabhaVati for 28 days was isolated and studied for the genome-wide changes in the expression.

Results: The results revealed several molecular targets that could contribute to the therapeutic effects of ChandraprabhaVati. Several genes have been differentially expressed, among those miRNAs miR-434, miR877, and miRlet7e contribute to the anti-diabetic, anti-fibrotic and anti-inflammatory of CPV. The rejuvenative activity of CPV may be due to the MeOX1 and Upf3b genes. Up-regulation of Hbaa2 gene facilitates the anti-anemic effect. Interestingly gender-specific differential expressions of genes were also observed. Rab3d were found to be altered in female when compared to male animals.

Conclusion: Thus the microarray data for the CPV treated animals has revealed molecular targets that may be responsible for the various known therapeutic effects and also identified new beneficial effects of CPV.

Keywords: Ayurveda; ChandraprabhaVati; Microarray analysis.

PubMed Disclaimer

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Hierarchical clustering of gene expression levels in the liver samples of animals from different groups treated with CPV.
Fig. 2
Fig. 2
Volcano plots for control versus medium dose of CPV. [A] Control versus medium dose of CPV; [B] Honey-administered versus medium dose of CPV.
Fig. 3
Fig. 3
Volcano plots for [A] normal versus high dose of CPV treated group, [B] honey versus high dose of CPV treated samples, [C] medium dose versus high dose of CPV treated groups.
Fig. 4
Fig. 4
; Differential gene expression of therapeutic, medium and high dose treated groups. A. Venn diagram for treated groups:50 mg/kg; 200 mg/kg; 800 mg/kg; B. Number of genes down regulated.
Fig. 5
Fig. 5
Gene expression analysis using RT-PCR. The relative gene expressions was depicted in fold change. *p < 0.05.
Fig. 6
Fig. 6
Pathways altered by ChandraprabhaVati (CPV).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Tarkang P.A., Appiah-Opong R., Ofori M.F., Ayong L.S., Nyarko A.K. Application of multi-target phytotherapeutic concept in malaria drug discovery: a systems biology approach in biomarker identification. Biomark Res. 2016;4(1):25. - PMC - PubMed
    1. Li C.-C., Lo H.-Y., Hsiang C.-Y., Ho T.-Y. DNA microarray analysis as a tool to investigate the therapeutic mechanisms and drug development of Chinese medicinal herbs. Biomedicine. 2012;2(1):10–16.
    1. Waring J.F., Gum R., Morfitt D. Identifying toxic mechanisms using DNA microarrays: evidence that an experimental inhibitor of cell adhesion molecule expression signals through the aryl hydrocarbon nuclear receptor. Toxicology. 2002;181–182:537–550. doi: 10.1016/S0300-483X(02)00477-8. - DOI - PubMed
    1. KiyamaR DNA microarray-based screening and characterization of traditional Chinese medicine. Microarrays. 2017;6(1):4.
    1. Wen Z., Wang Z., Wang S. Discovery of molecular mechanisms of traditional Chinese medicinal formula Si-Wu-Tang using gene expression Microarray and Connectivity Map. PLoS One. 2011;6(3) - PMC - PubMed

LinkOut - more resources