Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells
- PMID: 31958452
- DOI: 10.1016/j.jim.2020.112748
Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells
Abstract
Gene silencing using small interfering ribonucleic acids (siRNA) is a powerful method to interfere with gene expression, allowing for the functional exploration of specific genes. siRNA interference can be applied in both cell lines, as well as in primary, non-dividing cell types like dendritic cells. However, the efficacy in different cell types is variable and requires optimization. Here, we showed that the type of culture medium used during lipid-based siRNA-mediated transfection acts as a critical factor, affecting dendritic cell activation. Transfection of immature monocyte-derived dendritic cells in RPMI medium, but not in IMDM, showed increased transcript levels of pro-inflammatory cytokines. Moreover, the expression of co-stimulatory molecules was enhanced, thereby increasing the T cell stimulatory capacity. Our data demonstrates that the choice of medium should be critically examined as one of the variables while optimizing cell transfection.
Keywords: Cell maturation; Dendritic cells; RNA interference; Transfection; siRNA.
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
Conflict of interest statement
Disclosure None.
Comment in
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Innate IRE1α-XBP1 activation by viral single-stranded RNA and its influence on lung cytokine production during SARS-CoV-2 pneumonia.Genes Immun. 2024 Feb;25(1):43-54. doi: 10.1038/s41435-023-00243-6. Epub 2023 Dec 25. Genes Immun. 2024. PMID: 38146001
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