Publisher Correction: The glycogen synthase kinase MoGsk1, regulated by Mps1 MAP kinase, is required for fungal development and pathogenicity in Magnaporthe oryzae

Abstract

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Figure 1

Plant infection assays and microscopic observation on infection process of the Δmogsk1 mutant. (A) Appressoria of Ku80 and Δmogsk1 were induced at the hyphal tips following 48 h inoculation on hydrophobic cover slips at a moisture chamber at room temperature. Bar = 10 μm. (B) Microscopic observation on mycelial plug inoculated area on unwounded barley leaf tissues 48 hr post inoculation. Bar = 10 μm. (C) Equal amount of mycelial plugs from Ku80, Δmogsk1 and Δmogsk1/MoGSK1 were inoculated on 15-day-old rice seedlings (CO39). Photos were taken post 5-day inoculation. (D) Disease symptoms on wounded and unwounded 7-day-old susceptible barley seedlings induced by mycelia plugs of Ku80 and Δmogsk1 were photographed post 5-day inoculation.

Figure 2

Over-expression of MoGSK1 affects appressorium morphogenesis in M. oryzae. (A) RNA gel blot showing induction of MoGSK1 (Line 1) in the transformant expressing P_MPG1:MoGSK1 compared to Guy11. (B) Microscopic observation of appressorium morphology induced on hydrophobic cover slips for 24 hr in the transformant expressing P_MPG1:MoGSK1. Bar = 10 μm. (C) Penetration assay to demonstrate pathogenicity of the MoGSK1 overexpression strain. Appressorium formation (24 hr) and penetration hyphae (48 hr) developed on plant surface are shown in left and right hand panels. Bar = 10 μm.