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. 1988 Oct 1;255(1):357-60.

Inhibition by cyclosporin A of a Ca2+-dependent pore in heart mitochondria activated by inorganic phosphate and oxidative stress

M Crompton  1 , H Ellinger, A Costi
Affiliations

Inhibition by cyclosporin A of a Ca2+-dependent pore in heart mitochondria activated by inorganic phosphate and oxidative stress

M Crompton et al. Biochem J. .

Abstract

The capacity of cyclosporin A to inhibit opening of a Ca2+-dependent pore in the inner membrane of heart mitochondria was investigated. Whereas in the presence of 25 nmol of Ca2+/mg of mitochondrial protein and 5 mM-Pi mitochondria were unable to maintain accumulated Ca2+, inner-membrane potential and sucrose impermeability, all three parameters were preserved when cyclosporin was included. Pore opening was assayed directly by [14C]sucrose entry and entrapment in the matrix space. [14C]Sucrose entry induced by both Ca2+ plus Pi and Ca2+ plus t-butyl hydroperoxide was almost completely inhibited by 60 pmol of cyclosporin/mg of mitochondrial protein. It is concluded that cyclosporin A is a potent inhibitor of the pore.

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References

    1. FEBS Lett. 1980 Sep 22;119(1):1-8 - PubMed
    1. J Mol Cell Cardiol. 1982 May;14(5):267-77 - PubMed
    1. Biochem J. 1983 Nov 15;216(2):333-42 - PubMed
    1. J Mol Cell Cardiol. 1984 Feb;16(2):175-87 - PubMed
    1. Transplantation. 1984 Aug;38(2):161-4 - PubMed

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