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. 2020 Dec;58(1):124-130.
doi: 10.1080/13880209.2019.1711430.

Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds

Aitian Zheng  1 , Huadan Ma  1 , Xianbin Liu  1 , Qing Huang  1 , Zheng Li  1 , Lin Wang  1 , Na Zeng  1 , Biaoliang Wu  1
Affiliations

Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds

Aitian Zheng et al. Pharm Biol. 2020 Dec.

Abstract

Context: Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healing by regulating autophagy.Objective: To explore the mechanism of moist exposed MEBT/MEBO promoting diabetic wound repair.Materials and methods: Eighty male Wistar rats were randomly assigned to control (n = 20) and diabetic group induced by intraperitoneal injection of STZ (n = 60), which were further randomly assigned to MEBO, Kangfuxin and model groups (n = 20 each). All rats underwent full-thickness skin resection in the back. Wound healing was dynamically observed and wound tissues were collected at five time points for pathological examination, autophagosome and the expression of PI3K, Akt and mTOR.Results: The healing time in the control group was the shortest, no statistically significant difference was found between the MEBO and the Kangfuxin group (p = 0.76). The morphology of autophagosomes ranged large to small, which was the most obvious in the MEBO group. The mRNA and protein expression of PI3K, Akt and mTOR in each group reached the peak on Day 5, the levels in the MEBO group were the highest (F = 18.43, 19.97, 15.36, p < 0.05). On Day 11, the expression levels in each group began to decline.Discussion and conclusions: In this study, we discussed the molecular mechanism of MEBT/MEBO promoting the repair of diabetic ulcer wounds through autophagy and PI3K-Akt-mTOR signalling pathway, which provides a new way for drug design in the future.

Keywords: Diabetes; PI3K–Akt–mTOR; fibroblasts; traditional Chinese medicine; wound healing rate.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1.
Figure 1.
Comparison of healing of wounds in different groups at different time points.
Figure 2.
Figure 2.
HE staining of wound tissue in different groups at different time points.
Figure 3.
Figure 3.
Autophagosome formation in wound tissue in different groups at different time points.
Figure 4.
Figure 4.
Expression of PI3K, Akt and mTOR mRNA in different groups at different time points. ap < 0.05, compared with the control group; bp < 0.05, compared with the model group; cp < 0.05, compared with the Kangfuxin group.
Figure 5.
Figure 5.
Expression of PI3K, P-PI3K, Akt and mTOR proteins on wound tissue in different groups at different time points ap < 0.05, compared with the control group; bp < 0.05, compared with the model group; cp < 0.05, compared with the rehabilitation group.
See this image and copyright information in PMC

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