In vivo electrochemical detection of 5-hydroxyindole within the trigeminal nucleus caudalis of freely moving rats: the effect of morphine

Abstract

The trigeminal nucleus caudalis is considered the equivalent of the orofacial nociceptive system of the dorsal horn of the spinal cord. At the level of this trigeminal area (i.e. medullary dorsal horn) the transmission of noxious inputs is strongly modulated by a descending, serotonergic system mainly originating from the nucleus raphe magnus (NRM). The present study in freely moving animals reports the effect of morphine on the 5-hydroxyindole oxidation current recorded in the medullary dorsal horn. Complementary data from recordings in spinal dorsal horn in acutely anesthetized rats are also presented. A current recorded at 270-290 mV (peak '3'), characteristic of 5-hydroxyindoleacetic acid (5-HIAA), was measured with treated multi-fiber carbon electrodes, using differential pulse voltammetry (DPV) or differential normal pulse voltammetry (DNPV). In control rats, the amplitude of the peak remained constant for many hours. Morphine (10 mg/kg i.p.) caused a significant increase which plateaued between 35 and 80 min (mean increase: 127 +/- 5% of control values); recovery was complete by about 3 h. Simultaneous injection of naloxone (1 mg/kg i.p.) totally abolished the effect of morphine. By contrast, morphine was without effect on peak 3 recorded in the spinal dorsal horn of chloral hydrate (450 mg/kg i.p.) anesthetized rats. It is concluded that in non-anesthetized freely moving animals morphine clearly increases the metabolism of serotonin (5-HT) in the medullary dorsal horn. This finding confirms previous neurochemical data showing an increased synthesis or release of 5-HT in the spinal cord after systemic morphine or its microinjection into either the periaqueductal gray matter or the NRM, and underlines the value of in vivo electrochemistry in monitoring changes in 5-HT metabolism directly and continuously during various physiological and pharmacological procedures.