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Review
. 2020 Jan 21;21(3):708.
doi: 10.3390/ijms21030708.

An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells

Dinh-Toi Chu  1   2 , Thuy Nguyen Thi Phuong  3 , Nguyen Le Bao Tien  4 , Dang Khoa Tran  5 , Vo Van Thanh  4   6 , Thuy Luu Quang  7 , Dang Tien Truong  8 , Van Huy Pham  9 , Vo Truong Nhu Ngoc  2 , Thien Chu-Dinh  10 , Kushi Kushekhar  11
Affiliations
Review

An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells

Dinh-Toi Chu et al. Int J Mol Sci. .

Abstract

Bone marrow mesenchymal stem/stromal cells (BMSCs), which are known as multipotent cells, are widely used in the treatment of various diseases via their self-renewable, differentiation, and immunomodulatory properties. In-vitro and in-vivo studies have supported the understanding mechanisms, safety, and efficacy of BMSCs therapy in clinical applications. The number of clinical trials in phase I/II is accelerating; however, they are limited in the size of subjects, regulations, and standards for the preparation and transportation and administration of BMSCs, leading to inconsistency in the input and outcome of the therapy. Based on the International Society for Cellular Therapy guidelines, the characterization, isolation, cultivation, differentiation, and applications can be optimized and standardized, which are compliant with good manufacturing practice requirements to produce clinical-grade preparation of BMSCs. This review highlights and updates on the progress of production, as well as provides further challenges in the studies of BMSCs, for the approval of BMSCs widely in clinical application.

Keywords: bone marrow mesenchymal stem cells; clinical application; culture; isolation; stem cell therapy; storage.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Map of clinical trials on bone marrow stem cells in the world [27]. North America comprises the highest number of trials, followed by Europe, the Middle East, and East Asia.
Figure 2
Figure 2
The process of bone marrow mesenchymal stem cell transplantation.
Figure 3
Figure 3
Summarize the current clinical applications of bone marrow mesenchymal stem cells.
See this image and copyright information in PMC

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