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. 2020 Jan 21;10(2):166.
doi: 10.3390/biom10020166.

The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production

Robert Duliński  1 , Marek Zdaniewicz  2 , Aneta Pater  2 , Dagmara Poniewska  1 , Krzysztof Żyła  1
Affiliations

The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production

Robert Duliński et al. Biomolecules. .

Abstract

A relatively high concentration of phytate in buckwheat malt, and the low activity of endogenous buckwheat phytases, both of which limit the effective use of substrates (starch, proteins, minerals) for fermentation and yeast metabolism, gives rise to the potential for application of phytases in beer production. This study aims at obtaining a 100% buckwheat wort with high bioactive cyclitols (myo-inositol and D-chiro-inositol) concentrations released by exogenous phytases and acid phosphatases. Two mashing programs were used in the study, i.e., (1) typical for basic raw materials, namely the well-established Congress method, and (2) optimized for phytase activity. The results indicated a nearly 50% increase in the level of bioactive myo-inositol and an 80% degradation of phytate in the wort as a result of simultaneous application of phytase and phosphatase enzymes in the mashing of buckwheat malt. In addition, high D-chiro-inositol concentrations were released from malt to the buckwheat wort. The concerted action of the two phytases significantly increased (19-44%) Zn2+ concentrations in wort. This may be of great importance during mash fermentation by Saccharomyces cerevisiae yeasts. There is a potential to develop technology for buckwheat beer production, which, in addition to being free from gluten, comprises high levels of bioactive myo- and D-chiro-inositols.

Keywords: D-chiro-inositol; HPAEC-PAD; buckwheat beer; inositol phosphates profile; myo-inositol.

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Conflict of interest statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Figures

Figure 1
Figure 1
Profile of the inositol phosphates by HPAEC–UV/Vis chromatography on CarboPackPA1 column obtained from the analysis of the buckwheat wort using the mashing program optimized for the activity of phytases.
Figure 2
Figure 2
Sample chromatogram from the profile in the control buckwheat wort analyzed by HPAEC–UV/Vis chromatography.
See this image and copyright information in PMC

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