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. 2015 Sep;80(9):1380-1383.
doi: 10.1002/cplu.201500156. Epub 2015 Jun 10.

Cyclopamine-Paclitaxel-Containing Nanoparticles: Internalization in Cells Detected by Confocal and Super-Resolution Microscopy

Gaia Fumagalli  1 , Davide Mazza  2 , Michael S Christodoulou  1 , Giovanna Damia  3 , Francesca Ricci  3 , Dario Perdicchia  1 , Barbara Stella  4 , Franco Dosio  4 , Panagiota A Sotiropoulou  5 , Daniele Passarella  1
Affiliations

Cyclopamine-Paclitaxel-Containing Nanoparticles: Internalization in Cells Detected by Confocal and Super-Resolution Microscopy

Gaia Fumagalli et al. Chempluschem. 2015 Sep.

Abstract

Cyclopamine- and paclitaxel-containing hetero-nanoparticles generated by self-assembly show combined efficacy in the treatment of three different cancer cell lines. The use of ternary combination with the addition of a dye-squalene conjugate secured the obtainment of fluorescent nanoparticles that permitted the observation of the cellular internalization by confocal microscopy and super-resolution dSTORM (direct stochastic optical reconstruction microscopy).

Keywords: cancer stem cells; cyclopamine; nanoparticles; paclitaxel.

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References

    1. P. A. Sotiropoulou, M. S. Christodoulou, A. Silvani, C. Herold-Mende, D. Passarella, Drug Discovery Today 2014, 19, 1547-1562.
    1. Y. Zhou, J. Yang, J. S. Rhim, J. Kopeček, J. Controlled Release 2013, 172, 946-953.
    1. S. Singh, D. Chitkara, R. Mehrazin, S. W. Behrman, R. W. Wake, R. I. Mahato, PLoS One 2012, 7, e40021.
    1. H. Hillaireau, P. Couvreur, Cell. Mol. Life Sci. 2009, 66, 2873-2896.
    1. P. Couvreur, C. Vauthier, Pharm. Res. 2006, 23, 1417-1450.

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