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Comment
. 2020 Nov;69(11):2051-2052.
doi: 10.1136/gutjnl-2019-320399. Epub 2020 Jan 23.

Transgenic expression of human PRSS2 exacerbates pancreatitis in mice

Jianhua Wan  1 , Ashley Haddock  1 , Brandy Edenfield  1 , Baoan Ji  2 , Yan Bi  3
Affiliations
Comment

Transgenic expression of human PRSS2 exacerbates pancreatitis in mice

Jianhua Wan et al. Gut. 2020 Nov.
No abstract available

Keywords: experimental pancreatitis.

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Conflict of interest statement

Competing interests: None declared.

Figures

Figure 1
Figure 1
Caerulein-induced severe acute pancreatitis in mice with transgenic expression of human PRSS2. (A) Wild type (WT) C57BL6 control and transgenic mice was injected with a single dose of caerulein (100 μg/kg) intraperitoneally. Macroscopic appearance of pancreas 24 hours after caerulein induction in control and PRSS2 line #1 mice was shown. (B) Pancreatic oedema and serum amylase were measured and compared in control and PRSS2 line #1 mice (n=5–8). (C) Pancreas histology (H&E) and CD11b-positive inflammatory cell staining. (D) Pancreatitis histopathological scores were evaluated.
Figure 2
Figure 2
Transgenic PRSS2 mice but not WT C57BL control mice developed chronic pancreatitis after one episode of acute pancreatitis. (A) Schema of pancreatitis induction and tissue harvest time points. (B) Measurements of pancreas size at day 7 and day 21 after acute pancreatitis induction (n=6–8). (C) Pancreas histology (H&E) at day 7 and day 21 after caerulein.
See this image and copyright information in PMC

Comment on

References

    1. Hegyi E, Tóth AZ, Áron Vincze, et al. Alcohol-Dependent effect of Prss1-Prss2 haplotype in chronic pancreatitis. Gut 2020;69:1–2. - PMC - PubMed
    1. Gui F, Zhang Y, Wan J, et al. Trypsin activity governs increased susceptibility to pancreatitis in mice expressing human Prss1r122H. J Clin Invest 2019. - PMC - PubMed
    1. Jancsó Z, Sahin-Tóth M. Mutation that promotes activation of trypsinogen increases severity of secretagogue-induced pancreatitis in mice. Gastroenterology 2019. doi:10.1053/j.gastro.2019.11.020. [epub ahead of print: 18 Nov 2019]. - DOI - PMC - PubMed
    1. Guy O, Lombardo D, Bartelt DC, et al. Two human trypsinogens. Purification, molecular properties, and N-terminal sequences. Biochemistry 1978;17:1669–75. - PubMed
    1. Rinderknecht H, Stace NH, renner IG. Effects of chronic alcohol abuse on exocrine pancreatic secretion in man. Dig Dis Sci 1985;30:65–71. - PubMed

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