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. 2020:2122:223-237.
doi: 10.1007/978-1-0716-0342-0_16.

Microscopical Detection of Cell Death Processes During Scots Pine Zygotic Embryogenesis

Jaana Vuosku  1 , Suvi Sutela  2
Affiliations

Microscopical Detection of Cell Death Processes During Scots Pine Zygotic Embryogenesis

Jaana Vuosku et al. Methods Mol Biol. 2020.

Abstract

Programmed cell death (PCD) processes are essential in the plant embryogenesis. To understand how PCD operates in a developing seed, the dying cells need to be identified in relation to their surviving neighbors. This can be accomplished by the means of in situ visualization of fragmented DNA-a well-known hallmark of PCD. In the developing Scots pine (Pinus sylvestris L.) seed, several tissues die via morphologically different PCD processes during the embryogenesis. Here, we describe the protocols for the characterization of Scots pine seeds at the early and late developmental stages and, further, the localization of nucleic acids and DNA fragmentation by the acridine orange staining and TUNEL (terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labeling) assay in the dying seed tissues.

Keywords: Acridine orange staining; DNA fragmentation; Pinus; Programmed cell death; Scots pine; TUNEL assay; Zygotic embryogenesis.

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References

    1. Bozhkov PV, Filonova LH, Suarez MF (2005) Programmed cell death in plant embryogenesis. Curr Top Dev Biol 67:135–179 - DOI
    1. Earnshaw WC (1995) Nuclear changes in apoptosis. Curr Opin Cell Biol 7:337–343 - DOI
    1. Yamada T, Takatsu Y, Kasumi M et al (2006) Nuclear fragmentation and DNA degradation during programmed cell death in petals of morning glory (Ipomoea nil). Planta 224:1279–1290 - DOI
    1. Vuosku J, Sarjala T, Jokela A et al (2009) One tissue, two fates: different roles of megagametophyte cells during scots pine embryogenesis. J Exp Bot 60:1375–1386 - DOI
    1. Gavrieli Y, Sherman Y, Bensasson SA (1992) Identification of programmed cell-death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493–501 - DOI

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