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. 2020 Jan 24;15(1):e0227929.
doi: 10.1371/journal.pone.0227929. eCollection 2020.

HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system

Woottichai Khamduang  1   2   3 , Ampika Kaewbundit  3 , Amonrat Duangmano  3 , Sayamon Hongjaisee  4 , Virat Klinbuayaem  5 , Guttiga Halue  6 , Apichat Chutanunta  7 , Wasna Sirirungsi  1 , Gonzague Jourdain  1   3   8 , Nicole Ngo-Giang-Huong  1   3   8
Affiliations

HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system

Woottichai Khamduang et al. PLoS One. .

Abstract

World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log10 copies/mL. Using the 1,000 copies/mL cut-off, the sensitivity of DBS was 97% (95%CI, 91-100%) and specificity was 75% (95%CI, 19-99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.

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Conflict of interest statement

All authors have declared no conflict of interest.

Figures

Fig 1
Fig 1
(A) Regression analysis of HIV RNA levels in paired plasma and DBS specimens from 80 participants. (B) Bland-Altman analysis of agreement between HIV RNA levels in 80 paired plasma and DBS specimen. The horizontal lines represent the mean difference and +2 standard deviations (SD).
See this image and copyright information in PMC

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