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Observational Study
. 2020 May;1865(5):158637.
doi: 10.1016/j.bbalip.2020.158637. Epub 2020 Jan 23.

A single nucleotide polymorphism of PLIN2 is associated with nonalcoholic steatohepatitis and causes phenotypic changes in hepatocyte lipid droplets: A pilot study

Claire S Faulkner  1 , Collin M White  2 , Vijay H Shah  3 , Loretta L Jophlin  4
Affiliations
Observational Study

A single nucleotide polymorphism of PLIN2 is associated with nonalcoholic steatohepatitis and causes phenotypic changes in hepatocyte lipid droplets: A pilot study

Claire S Faulkner et al. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 May.
No abstract available

Keywords: Hepatic steatosis; Lipid droplet; Nonalcoholic steatohepatitis; Perilipin-2; Single nucleotide polymorphism.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1.
Fig. 1.
A) Representative electropherograms and distribution of PLIN2 genotypes in study subjects, stratified by control and NASH cohorts. B) Allelic, dominant and recessive inheritance models and odds ratios (OR) for NASH conveyed by the rs35568725, aka the minor allele (a). C) Fluorescence microscopy of representative single Huh7 hepatocytes expressing green fluorescent protein (GFP) tagged-PLIN2 (left) and GFP-PLIN2Pro251 (right) localized to LDs treated with vehicle (top) and 0.8 μm oleic acid for 48 h (bottom), scale bar = 10 μm. D) LDs per cell (left) and LD diameter (middle) quantified from ≥10 cells with similar GFP-PLIN2 and GFP-PLIN2Pro251 expression levels under all experimental conditions from 3 independent experiments (N = 3) and triglyceride levels (right) of steatotic flow cytometry-sorted GFP-positive Huh7 hepatocytes expressing GFP-PLIN2 versus GFP-PLIN2Pro251 normalized to total cellular protein (N = 4). Veh, vehicle treatment; OA, oleic acid 0.8 mM 48 h; ns, not significant; error bar = 1 standard deviation. *p < 0.05.
See this image and copyright information in PMC

References

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