Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jan 26;9(2):153.
doi: 10.3390/plants9020153.

Application of Deep Eutectic Solvents for the Extraction of Rutin and Rosmarinic Acid from Satureja montana L. and Evaluation of the Extracts Antiradical Activity

Martina Jakovljević  1 , Jelena Vladić  2 , Senka Vidović  2 , Kristian Pastor  2 , Stela Jokić  1 , Maja Molnar  1 , Igor Jerković  3
Affiliations

Application of Deep Eutectic Solvents for the Extraction of Rutin and Rosmarinic Acid from Satureja montana L. and Evaluation of the Extracts Antiradical Activity

Martina Jakovljević et al. Plants (Basel). .

Abstract

Satureja montana L. was used in the current research as the plant exhibits numerous health-promoting benefits due to its specific chemical composition. The extraction method based on deep eutectic solvents (DESs) was used for the extraction of rutin and rosmarinic acid from this plant. Five different choline chloride-based DESs with different volumes of water (10%, 30%, and 50% (v/v)) were used for the extraction at different temperatures (30, 50, and 70 °C) to investigate the influence on rosmarinic acid and rutin content obtained by high-performance liquid chromatography with diode-array detector (HPLC-DAD) in the obtained extracts. A principal component analysis was employed to explore and visualize the influence of applied parameters on the efficiency of the extraction procedure of rutin and rosmarinic acid. Among the tested DESs, choline chloride:lactic acid (mole ratio 1:2) and choline chloride:levulinic acid (mole ratio 1:2) were the most suitable for the extraction of rutin, while for rosmarinic acid choline chloride:urea (mole ratio 1:2) was the most effective solvent. The extract showing the best antiradical activity was obtained with choline chloride:urea (mole ratio 1:1) at 30 °C and 50% H2O (v/v).

Keywords: Satureja montana L., principal component analysisn/a; deep eutectic solvents; extraction; rosmarinic acid; rutin.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Principal component analysis (PCA) biplot of the extracts with scores representing various H2O content (10%, 30%, and 50% (v/v)) and pure water utilized in the extraction procedure.
Figure 2
Figure 2
Biplot of the extracts with scores representing various temperatures (30, 50, and 70 °C) utilized in the extraction procedure.
Figure 3
Figure 3
PCA biplot of the extracts with scores representing various solvent types (ChCl-U, ChCl-Sor, ChCl-BDO, ChCl-Lac, ChCl-LeA, and pure H2O) utilized in the extraction procedure.
Figure 4
Figure 4
The antiradical activity of the samples shown as percentage inhibition of DPPH (a-temperature at 30 °C; b a-temperature at 50 °C; c-temperature at 70 °C).
See this image and copyright information in PMC

References

    1. Zainal-Abidin M.H., Hayyan M., Hayyan A., Jayakumar N.S. New horizons in the extraction of bioactive compounds using deep eutectic solvents: A review. Anal. Chim. Acta. 2017;979:1–23. doi: 10.1016/j.aca.2017.05.012. - DOI - PubMed
    1. Bakirtzi C., Triantafyllidou K., Makris D.P. Novel lactic acid-based natural deep eutectic solvents: Efficiency in the ultrasound-assisted extraction of antioxidant polyphenols from common native Greek medicinal plants. J. Appl. Res. Med. Aromat. Plants. 2016;3:120–127. doi: 10.1016/j.jarmap.2016.03.003. - DOI
    1. Abbott A.P., Capper G., Davies D.L., Rasheed R.K., Tambyrajah V. Novel solvent properties of choline chloride/urea mixtures. Chem. Commun. 2003;70–71 doi: 10.1039/b210714g. - DOI - PubMed
    1. Abbott A.P., Boothby D., Capper G., Davies D.L., Rasheed R.K. Deep eutectic solvents formed between choline chloride and carboxylic acids: Versatile alternatives to ionic liquids. J. Am. Chem. Soc. 2004;126:9142–9147. doi: 10.1021/ja048266j. - DOI - PubMed
    1. Radošević K., Ćurko N., Gaurina Srček V., Cvjetko Bubalo M., Tomašević M., Kovačević Ganić K., Radojčić Redovniković I. Natural deep eutectic solvents as beneficial extractants for enhancement of plant extracts bioactivity. LWT- Food Sci. Technol. 2016;73:45–51. doi: 10.1016/j.lwt.2016.05.037. - DOI

LinkOut - more resources