Zwiesel bat banyangvirus, a potentially zoonotic Huaiyangshan banyangvirus (Formerly known as SFTS)-like banyangvirus in Northern bats from Germany

Abstract

Bats are reservoir hosts for several emerging and re-emerging viral pathogens causing morbidity and mortality in wildlife, animal stocks and humans. Various viruses within the family Phenuiviridae have been detected in bats, including the highly pathogenic Rift Valley fever virus and Malsoor virus, a novel Banyangvirus with close genetic relation to Huaiyangshan banyangvirus (BHAV)(former known as Severe fever with thrombocytopenia syndrome virus, SFTSV) and Heartland virus (HRTV), both of which have caused severe disease with fatal casualties in humans. In this study we present the whole genome of a novel Banyangvirus, named Zwiesel bat banyangvirus, revealed through deep sequencing of the Eptesicus nilssonii bat virome. The detection of the novel bat banyangvirus, which is in close phylogenetic relationship with the pathogenic HRTV and BHAV, underlines the possible impact of emerging phenuiviruses on public health.

Figure 1

Phylogenetic analysis of the nucleotide sequence (6,022 nt) of the Zwiesel bat banyangvirus L segment). Alignments were built with ClustalW before model prediction with JModel-test. Phylogenetic tree reconstruction was calculated using MrBayes with 500,000 replicates, model GTR, sampling frequency 200, burn in 10% (50,000). Posterior probabilities are depicted at the branches.

Figure 2

Phylogenetic analysis of the nucleotide sequence (3,094 nt) of the Zwiesel bat banyangvirus M segment. Alignments were built with ClustalW before model prediction with JModel-test. Phylogenetic tree reconstruction was calculated using MrBayes with 500,000 replicates, model GTR, sampling frequency 200, burn in 10% (50,000). Posterior probabilities are depicted at the branches.

Figure 3

Phylogenetic analysis of the nucleotide sequence (1,793 nt) of the Zwiesel bat banyangvirus S segment. Alignments were built with ClustalW before model prediction with JModel-test. Phylogenetic tree reconstruction was calculated using MrBayes with 500,000 replicates, model GTR, sampling frequency 200, burn in 10% (50,000). Posterior probabilities are depicted at the branches.