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. 2020 Jan 14:9:461.
doi: 10.3389/fcimb.2019.00461. eCollection 2019.

Serum IgG Anti- Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

Fabiana Nakashima  1 , Valquíria Sousa Pardo  1 , Marcos Paulo Miola  1   2 , Fernando Henrique Antunes Murata  3 , Natalia Paduan  1 , Stefani Miqueline Longo  1 , Cinara Cássia Brandão de Mattos  1   3 , Vera Lucia Pereira-Chioccola  4 , Octávio Ricci Jr  2 , Luiz Carlos de Mattos  1   3
Affiliations

Serum IgG Anti- Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

Fabiana Nakashima et al. Front Cell Infect Microbiol. .

Abstract

Background:Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-T. gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t-test or Mann-Whitney according to the normal distribution (p-value ≤ 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM+/IgG-: 5 (0.6%); IgM+/IgG+: 21 (2.8%); IgM-/IgG+: 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions: In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors.

Keywords: Nested PCR; Toxoplasma gondii; blood donation and transfusion; blood donnors; molecular diagnosis; serology; serology assay; transfusion.

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Figures

Figure 1
Figure 1
Electrophoretic profile of fragments of B1 gene from T. gondii genomic DNA extracted from peripheral blood of blood donors, amplified by Nested PCR. In (A), the first amplification is showing a fragment of 288 base pairs; in (B), the second amplification is showing a fragment of 115 base pairs. M, molecular marker; PC, Positive Control; CN, Negative Control.
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