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. 2020 Apr 9;50(2):455-463.
doi: 10.3906/sag-1803-196.

Effect of metformin and detorsion treatment on serum anti-Müllerian hormonelevels and ovarian histopathology in a rat ovarian torsion model

Sema Karakaş  1 , Cihan Kaya  2 , Hakan Güraslan  2 , Damlanur Sakiz  3 , Sema Süzen Çaypinar  2 , Hüseyin Cengiz  3 , Murat Ekin  2 , Levent Yaşar  2
Affiliations

Effect of metformin and detorsion treatment on serum anti-Müllerian hormonelevels and ovarian histopathology in a rat ovarian torsion model

Sema Karakaş et al. Turk J Med Sci. .

Abstract

Background/aim: Adnexal torsion is a common gynaecological emergency, and considered to be a problem mostly in reproductive-age women. To evaluatethe effect of metformin and detorsion treatment on reducing ovarian reserve in an ovarian torsion model.

Materials and methods: Twenty-four nonpregnant, Wistar Hannover rats were included in the study. Animals were divided into 3 groups: the control group, the detorsion only group, and the metformin + detorsion group. The first group received only laparotomy. In the second group, ovaries were fixed to the abdominal wall after performing 360° ovarian torsion, followed by detorsion after a 3-h period of ischemia. The third group underwent the same torsion and detorsion procedures as the second group, and received 50 mg/kg metformin by gavage for 14 days. Ovarian damage scores, follicle counts, and AMH levels were evaluated.

Results: The total damage score was significantly increased in the detorsion only group compared to the metformin+detorsion and control groups. Pre-operative/post-operative AMH decreases were statistically significant in negative direction in the detorsion only group when compared to the metformin+detorsion and control groups (P = 0.001).

Conclusion: Metformin+detorsion treatment may be effective in protecting the ovarian reserve after ovarian torsion.

Keywords: anti-Müllerian hormone; detorsion; metformin; ovarian reserve; ovarian torsion.

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Conflict of interest statement

CONFLICT OF INTEREST:

none declared

Figures

Figure 1
Figure 1
The black arrow shows exposed ovary and uterine horn. The adnexa is rotated 360° clockwise, and then sutured to the abdominal wall.
Figure 2
Figure 2
The black arrow shows torsioned ovary after 3h ischemia period.
Figure 3
Figure 3
The black arrows show haemorrhage area with red blood cells. H and Ex200
Figure 4
Figure 4
The black arrows show degenerated granulosa cells. H and Ex200
See this image and copyright information in PMC

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