Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Apr 9;50(2):455-463.
doi: 10.3906/sag-1803-196.

Effect of metformin and detorsion treatment on serum anti-Müllerian hormonelevels and ovarian histopathology in a rat ovarian torsion model

Sema Karakaş  1 , Cihan Kaya  2 , Hakan Güraslan  2 , Damlanur Sakiz  3 , Sema Süzen Çaypinar  2 , Hüseyin Cengiz  3 , Murat Ekin  2 , Levent Yaşar  2
Affiliations

Effect of metformin and detorsion treatment on serum anti-Müllerian hormonelevels and ovarian histopathology in a rat ovarian torsion model

Sema Karakaş et al. Turk J Med Sci. .

Abstract

Background/aim: Adnexal torsion is a common gynaecological emergency, and considered to be a problem mostly in reproductive-age women. To evaluatethe effect of metformin and detorsion treatment on reducing ovarian reserve in an ovarian torsion model.

Materials and methods: Twenty-four nonpregnant, Wistar Hannover rats were included in the study. Animals were divided into 3 groups: the control group, the detorsion only group, and the metformin + detorsion group. The first group received only laparotomy. In the second group, ovaries were fixed to the abdominal wall after performing 360° ovarian torsion, followed by detorsion after a 3-h period of ischemia. The third group underwent the same torsion and detorsion procedures as the second group, and received 50 mg/kg metformin by gavage for 14 days. Ovarian damage scores, follicle counts, and AMH levels were evaluated.

Results: The total damage score was significantly increased in the detorsion only group compared to the metformin+detorsion and control groups. Pre-operative/post-operative AMH decreases were statistically significant in negative direction in the detorsion only group when compared to the metformin+detorsion and control groups (P = 0.001).

Conclusion: Metformin+detorsion treatment may be effective in protecting the ovarian reserve after ovarian torsion.

Keywords: anti-Müllerian hormone; detorsion; metformin; ovarian reserve; ovarian torsion.

PubMed Disclaimer

Conflict of interest statement

CONFLICT OF INTEREST:

none declared

Figures

Figure 1
Figure 1
The black arrow shows exposed ovary and uterine horn. The adnexa is rotated 360° clockwise, and then sutured to the abdominal wall.
Figure 2
Figure 2
The black arrow shows torsioned ovary after 3h ischemia period.
Figure 3
Figure 3
The black arrows show haemorrhage area with red blood cells. H and Ex200
Figure 4
Figure 4
The black arrows show degenerated granulosa cells. H and Ex200
See this image and copyright information in PMC

References

    1. Huchon C Fauconnier A. Adnexal torsion: a literature review. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2010;150:8. - PubMed
    1. Vijayalakshmi K Reddy GM Subbiah VN Sathiya S Arjun B Clinico-pathological profile of adnexal torsion cases: a retrospective analysis from a tertiary care teaching hospital. Journal of Clinical and Diagnostic Research. 2014;8:OC04. - PMC - PubMed
    1. Hibbard LT Adnexal torsion. American Journal of Obstetrics & Gynecology. 1985;152:456. - PubMed
    1. Houry D Abbott JT Ovarian torsion: a fifteen-year review. Annals of Emergency Medicine. 2001;38:156. - PubMed
    1. Genç M Şahin N Karaarslan S Güçlü S. The evaluation of clinical, radiological, pathological profile of ovarian torsion cases. Yeni Tıp Dergisi. 2015;32:79.

LinkOut - more resources