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. 1988 Dec 15;336(6200):651-6.
doi: 10.1038/336651a0.

Enhanced protein thermostability from designed mutations that interact with alpha-helix dipoles

H Nicholson  1 , W J Becktel, B W Matthews
Affiliations

Enhanced protein thermostability from designed mutations that interact with alpha-helix dipoles

H Nicholson et al. Nature. .

Abstract

Two different genetically engineered amino-acid substitutions designed to interact with alpha-helix dipoles in T4 lysozyme are shown to increase the thermal stability of the protein. Crystallographic analyses of the mutant lysozyme structures suggest that the stabilization is due to electrostatic interaction and does not require precise hydrogen bonding between the substituted amino acid and the end of the alpha-helix.

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