Corrigendum: Nm-seq maps 2'- O-methylation sites in human mRNA with base precision

Abstract

[This corrects the article PMC5712428.].

Figure 1 |

Nm-seq, based on oxidative cleavage for mapping 2’-O-methylation. (a) Schematic illustration of revised Nm-seq. Following eight rounds of OED, 5’ phosphorylation and the last OE, 3’- and 5’-adaptor ligation generate two kinds of RT templates: (i) 3’ monophosphates (block cross), which block 3’ ligation, and (ii) 3’-Nm (red triangle) fragments, which ligate with both 3’ and 5’ adaptors. Mispriming may occur if the 3’ end of excess RT primer hybridizes to 5’-ligated RNA, which would exclude the introduced in-line barcode. Correct priming would include the in-line barcode, which enables filtering off mispriming reads. (b) Metagene profile of Nm site distribution along mRNA transcript in HeLa cells. Y-axis represents the density of identified Nm sites along transcripts. (c) Distribution of Nm sites in HeLa mRNA among different amino acid codons. (d) Um is the dominant Nm modification in both HeLa mRNA with a depletion of A flanking the modification site. The gray ring represents the nucleotide distribution of Nm site, the inside ring represents the nucleotide distribution at the −1 position (5’) of Um, and the outside ring represents the nucleotide percentage at the +1 position (3’).