Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Jan 17:6:334.
doi: 10.3389/fmed.2019.00334. eCollection 2019.

Intestinal Organoids as a Tool for Inflammatory Bowel Disease Research

Hamish C K Angus  1 , A Grant Butt  2 , Michael Schultz  3 , Roslyn A Kemp  1
Affiliations
Review

Intestinal Organoids as a Tool for Inflammatory Bowel Disease Research

Hamish C K Angus et al. Front Med (Lausanne). .

Abstract

Inflammatory Bowel Diseases (IBD) are difficult to model as freshly acquired tissues are short-lived, provide data as a snapshot in time, and are not always accessible. Many patients with IBD are non-responders to first-line treatments, and responders are prone to developing resistance to treatment over time-resulting in reduced patient quality of life, increased time to remission, and potential relapse. IBD is heterogenous and we are yet to fully understand the mechanisms of disease; thus, our ability to diagnose and prescribe optimal treatment remains ineffective. Intestinal organoids are derived from patient tissues expanded in vitro. Organoids offer unique insight into individual patient disease and are a potential route to personalized treatments. However, organoid models do not contain functional microbial and immune cell components. In this review, we discuss immune cell subsets in the context of IBD, and the requirement of immune cell and microbial components in organoid models for IBD research.

Keywords: Inflammatory Bowel Disease; T cells; cytokines; diagnostics; immunology; organoid; regulation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Molodecky NA, Soon IS, Rabi DM, Ghali WA, Ferris M, Chernoff G, et al. . Increasing incidence and prevalence of the inflammatory Bowel diseases with time, based on systematic review. Gastroenterology. (2012) 142:46–54.e42; quiz e30. 10.1053/j.gastro.2011.10.001 - DOI - PubMed
    1. Ding NS, Hart A, De Cruz P. Systematic review: predicting and optimising response to anti-TNF therapy in Crohn's disease - algorithm for practical management. Aliment Pharmacol Ther. (2016) 43:30–51. 10.1111/apt.13445 - DOI - PubMed
    1. Sato T, Stange DE, Ferrante M, Vries RG, Van Es JH, Van den Brink S, et al. . Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology. (2011) 141:1762–72. 10.1053/j.gastro.2011.07.050 - DOI - PubMed
    1. Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, et al. . Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. (2011) 470:105–9. 10.1038/nature09691 - DOI - PMC - PubMed
    1. Sartor RB. Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis. Nat Clin Pract Gastroenterol Hepatol. (2006) 3:390–407. 10.1038/ncpgasthep0528 - DOI - PubMed