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. 2020 Jan-Feb;30(1):14-20.
doi: 10.4103/ijn.IJN_346_18. Epub 2019 Sep 6.

mtDNA Analysis: A Valuable Tool to Establish Relationships in Live Related Organ Transplants

Vikash C Mishra  1   2 , Kirti A Gautam  3 , Richa Chaudhary  1 , Hina Solanki  1 , Saurabh Pokhariyal  3 , Vishal Saxena  4 , Pranav Dorwal  5 , Trupti Deshpande  2 , Parvind Singh  1   2 , Archana Anthwal  1 , Nikki Dey  1 , Dinesh Chandra  1 , Vimarsh Raina  1   2
Affiliations

mtDNA Analysis: A Valuable Tool to Establish Relationships in Live Related Organ Transplants

Vikash C Mishra et al. Indian J Nephrol. 2020 Jan-Feb.

Abstract

Introduction: In India, 90% kidneys for transplantation are obtained from living donor while only 10% come from deceased donors. Since the rate of living organ donors is high, it therefore leads to the problem of organ trafficking.To minimize the chances of organ trafficking, the Transplantation of Human Organ Act (THOA) 2014 was enacted in India that makes it mandatory to prove the relationship between patient and donor by DNA testing. The present study was undertaken to evaluate the degree of matching between maternally related patients and donors, performed using mitochondrial DNA (mtDNA).

Methods: After taking an informed consent, a total of 84 subjects were recruited in the study, 42 kidney transplant recipients and 42 their corresponding donors. An attempt was made to establish and confirm the claimed relationship betweenrecipient and donor using mtDNA analysis.

Results: Out of the total 42 cases, mtDNA analysis supported the claimed relationship in 33 (78.57%) cases, whereas in 9 (21.42%) cases claimed relationship could not be supported.

Conclusion: mtDNA can be used as valuable tool to support the claimed relationships of maternal lineage. It is important that more and more organ transplant physicians, surgeons and committees are made aware of this diagnostic modality.

Keywords: Distant relationship; mtDNA analysis; relationship test; solid organ transplantation.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Mitochondrial Hyper Variable region I Forward Sequence
Figure 2
Figure 2
Mitochondrial Hyper Variable region I Reverse Sequence
Figure 3
Figure 3
Mitochondrial Hyper Variable region II Forward Sequence
Figure 4
Figure 4
Mitochondrial Hyper Variable region II Reverse Sequence
See this image and copyright information in PMC

References

    1. Government of India. Transplantation of human organs act, 1994. Central Act 42 of. 1994. [Last cited on 2018 Jun 08]. Available from: http//wwwmedindianet/tho/thobill1asp .
    1. Choo SY. The HLA system: Genetics, immunology, clinical testing, and clinical implications. Yonsei Med J. 2007;28(48):11–23. - PMC - PubMed
    1. Butler JM, Hill CR. Biology and genetics of new autosomal STR loci useful for forensic DNA analysis. Forensic Sci Rev. 2012;24:15–26. - PubMed
    1. Butler JM. Recent developments in Y-single tandem repeat and Y-single nucleotide polymorphism analysis. Forensic Sci Rev. 2003;15:91–111. - PubMed
    1. Giles RE, Blanc H, Cann HM, Wallace DC. Maternal inheritance of human mitochondrial DNA. Proc Natl Acad Sci U S A. 1980;77:6715–9. - PMC - PubMed