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. 2019 Dec 12:33:100633.
doi: 10.1016/j.nmni.2019.100633. eCollection 2020 Jan.

Anaerosphaera massiliensis sp. nov., a new bacterium isolated from the stool of a 39-year-old Pygmy

T Takakura  1 , R Francis  2   3 , H Anani  2   4 , M Bilen  2 , D Raoult  2   3 , J Y Bou Khalil  2
Affiliations

Anaerosphaera massiliensis sp. nov., a new bacterium isolated from the stool of a 39-year-old Pygmy

T Takakura et al. New Microbes New Infect. .

Expression of concern in

Abstract

Anaerosphaera massiliensis strain Marseille-P4592T (= CSURP4592T; = CCUG72452T) is a new species isolated from the stool of a 39-year-old male Pygmy from the Democratic Republic of Congo.

Keywords: Anaerosphaera massiliensis; culturomics; new species; stool; taxono-genomics.

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Figures

Fig. 1
Fig. 1
MALDI-TOF MS reference mass spectrum. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
Fig. 2
Fig. 2
Dendrogram based on the comparison of the Anaerosphaera massiliensis strain Marseille-P4592T MALDI-TOF reference spectrum (in red), 71 other species of the genus of Peptoniphilus and one from the genus Tissierella.
Fig. 3
Fig. 3
Scanning electron micrograph of Anaerosphaera massiliensis sp. nov. strain Marseille-P4592T using the Tabletop Microscope TM4000Plus from Hitachi. Scale bar and acquisition settings are shown on the original micrograph.
Fig. 4
Fig. 4
Phylogenetic tree showing the position of Anaerosphaera massiliensis sp. nov. strain Marseille P4592T relative to other phylogenetically close neighbours. The respective GenBank accession numbers for 16S rRNA genes are indicated in parentheses. Sequence alignments and phylogenetic inferences were obtained using the maximum likelihood method within MEGA 7 software. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 1000 times to generate a majority consensus tree.
Fig. 5
Fig. 5
Heatmap generated with OrthoANI values calculated using the OAT software between Anaerosphaera massiliensis sp. nov. and other closely related species with standing in nomenclature.
Fig. 6
Fig. 6
Phylogenetic tree showing the core genome-based phylogeny between Anaerosphaera massiliensis sp. nov. strain Marseille-P4592 (in red) and other closely related species with standing in nomenclature. The genomes were annotated with Prokka and these annotations were used by Roary with identity of 50% to identify the pangenome of strains, including 337 core genes, as previously described [16]. Phylogenetic inferences were obtained using the maximum likelihood method within MEGA 7 software. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 1000 times to generate a majority consensus tree.
See this image and copyright information in PMC

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