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. 2020 Aug;108(6):2417-2425.
doi: 10.1002/jbm.b.34574. Epub 2020 Feb 5.

Hyaluronic acid-heparin conjugated decellularized human great saphenous vein patches decrease neointimal thickness

Hualong Bai  1   2 , Zhiwei Wang  1 , Mingxing Li  1 , Yuanfeng Liu  1 , Wang Wang  3   2 , Peng Sun  1 , Shunbo Wei  1 , Zhiju Wang  3   2 , Jiang'an Li  4 , Alan Dardik  5   6
Affiliations

Hyaluronic acid-heparin conjugated decellularized human great saphenous vein patches decrease neointimal thickness

Hualong Bai et al. J Biomed Mater Res B Appl Biomater. 2020 Aug.

Abstract

Although the science of implantable materials has advanced therapeutic options in vascular surgery, graft failure is still a problem in need of a durable solution. With the development of coating and decellularization techniques, coated prosthetic grafts have become an option; however, whether decellularized human saphenous vein can be conjugated and implanted is not known. Human great saphenous vein (GSV) was harvested and decellularized and hyaluronic acid (HA)-heparin was conjugated to the GSV; water contact angles (WCA), morphology, and sulfur element change were measured before and after heparin bonding. GSV patches were implanted into the rat inferior vena cava and aorta; patches were harvested (Day 14) and analyzed. HA-heparin was successfully conjugated to the decellularized human GSV with altered morphology and reduced WCA. The HA-heparin coated decellularized GSV patch was anti-thrombotic in vitro, and significantly decreased neointimal thickness both in patch venoplasty and angioplasty in a rat model. Both CD90 and nestin positive cells participated in neointima formation. These data show that HA-heparin coated human GSV patches decrease neointimal thickness when used both in venoplasty and arterioplasty. Tissue engineered decellularized human GSV is a promising vascular prosthesis.

Keywords: angioplasty; great saphenous vein; heparin; macrophage; patch.

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References

REFERENCES

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