Sugar-Nanocapsules Imprinted with Microbial Molecular Patterns for mRNA Vaccination
- PMID: 32023415
- PMCID: PMC7286077
- DOI: 10.1021/acs.nanolett.9b03483
Sugar-Nanocapsules Imprinted with Microbial Molecular Patterns for mRNA Vaccination
Abstract
Innate immune cells recognize and respond to pathogen-associated molecular patterns. In particular, polysaccharides found in the microbial cell wall are potent activators of dendritic cells (DCs). Here, we report a new class of nanocapsules, termed sugar-capsules, entirely composed of polysaccharides derived from the microbial cell wall. We show that sugar-capsules with a flexible polysaccharide shell and a hollow core efficiently drain to lymph nodes and activate DCs. In particular, sugar-capsules composed of mannan (Mann-capsule) carrying mRNA (mRNA) promote strong DC activation, mRNA translation, and antigen presentation on DCs. Mann-capsules elicit robust antigen-specific CD4+ and CD8α+ T-cell responses with antitumor efficacy in vivo. The strategy presented in this study is generally applicable for utilizing pathogen-derived molecular patterns for vaccines and immunotherapies.
Keywords: dendritic cell; mRNA; nanoparticle; polysaccharide; vaccine.
Conflict of interest statement
The authors declare the following competing financial interest(s): O.C.F. has financial interest in Selecta Biosciences, Tarveda Therapeutics, and Seer.
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